FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535312
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95621.78624801984637No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT16760.3130884418806229No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT15690.2931000986340676No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA15000.2802104193442329No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT12720.2376184356039095No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12220.22827808829243507No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA11190.20903697283079772No Hit
CAATAGGCCTGTTAACGGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT10650.19894939773440534No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA10080.1883014017993245No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTGATGCCCTTGTATGCATGTAT10060.18792778790686554No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9960.18605971844457064No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAAT9900.1849388767671937No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAC9150.17092835579998206No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC8960.1673790238216218No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT8880.16588456825178588No Hit
CTTTCCAATCAATAGGCCTGTTAACGGGAAGTTTTCTAAAACAGTCTTTG8850.1653241474130974No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC8670.16196162238096662No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8250.1541157306393281No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7950.14851152225244343No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7730.14440176943539468No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACT7540.1408524374570344No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7360.1374899124249036No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTT7360.1374899124249036No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7300.1363690707475267No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6920.12927040679080612No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6740.12590788175867532No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6720.12553426786621633No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6660.12441342618883941No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6650.12422661924260993No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA6600.12329258451146247No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCG6520.12179812894162656No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG6480.1210509011567086No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT6480.1210509011567086No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6460.12067728726424963No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCTGG6400.1195564455868727No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6340.11843560390949577No Hit
AATATGGGCCTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTTGTCT6320.1180619900170368No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6280.11731476223211883No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6250.11675434139343038No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6130.11451265803867651No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAA6110.11413904414621753No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5980.11171055384523416No Hit
TTCTTCTACTGTGCCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC5960.11133693995277519No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5950.11115013300654572No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5940.11096332606031624No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5940.11096332606031624No Hit
GTTTTAGAAAACTTCCCGTTAACAGGCCTATTGATTGGAAAGTGTGTCAA5860.10946887049048033No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5760.10760080102818544No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGTTTT5710.10666676629703799No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG5710.10666676629703799No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5690.10629315240457901No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT5670.10591953851212003No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCAT5650.10554592461966106No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGA5640.10535911767343158No Hit
GACTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTG5610.1047986968347431No Hit
GAATACATGCATACAAGGGCATCAAAGCAGGATAACCACATTGTGTAAAA5610.1047986968347431No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5480.10237020653375975No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5470.10218339958753025No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5430.1014361718026123No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT5420.10124936485638282No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACT5410.10106255791015333No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT5380.10050213707146487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)