FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515746
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTAGA42690.8277330313759098No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17510.3395082075285121No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16110.3123630624377116No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14660.2842484478793825No Hit
CATTTACACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGGCCCACT13250.25690940889507624No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12490.2421734729886417No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11730.22743753708220713No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA11490.2227840836380699No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10940.21211991949525544No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10580.20513973932904955No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10400.20164964924594664No Hit
CACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT10350.20068017977841807No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10210.197965665269338No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9890.19176106067715504No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9400.18226025989537487No Hit
GTGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGGAT9130.17702512477072047No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9040.175280079729169No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC8960.17372892858112327No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT8830.17120830796554892No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8770.17004494460451464No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8490.16461591558635452No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8230.15957467435520586No Hit
CAATAACATAGCCCATGAAATGTAACGAGTAACCCCATCTTTTTGTTTTG7930.15375785755003432No Hit
CATGCATACAGGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC7860.1524006002954943No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7830.15181891861497715No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7810.1514311308279657No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7760.15046166136043712No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7760.15046166136043712No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA7760.15046166136043712No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7520.1458082079162999No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7440.14425705676825415No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG7240.14037917889813978No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCCTGTATGCATGTATT7160.13882802775009404No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC6840.13262342315791106No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACACC6750.1308783781163596No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6680.12952112086181958No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6650.12893943918130243No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6630.128551651394291No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT6490.12583713688521092No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6410.1242859857371652No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC6360.12331651626963659No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6310.12234704680210802No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT6300.12215315290860229No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGAA6300.12215315290860229No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCGTTACATTTCAT6240.12098978954756799No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6230.12079589565406226No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT6220.12060200176055656No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6200.1202142139735451No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC6150.11924474450601653No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC6080.11788748725147649No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6040.11711191167745362No Hit
CTCTATGACCGTTTCTCTCCCAAAAGTGAGACAAGAAATGTGAAACCACA6000.11633633610343076No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5960.11556076052940789No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5960.11556076052940789No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5860.1136218215943507No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5850.11342792770084498No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5790.11226456433981068No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5730.11110120097877636No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5650.10955004983073063No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTTCAAACC5630.10916226204371919No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5620.10896836815021348No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5610.10877447425670776No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCAGCCT5560.10780500478917918No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5540.10741721700216772No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5490.10644774753463915No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5480.10625385364113342No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA5480.10625385364113342No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5440.10547827806711055No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5410.10489659638659339No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5380.10431491470607623No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC5300.1027637635580305No Hit
GTGTGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGG5280.10237597577101908No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5280.10237597577101908No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT5240.1016004001969962No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5240.1016004001969962No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5240.1016004001969962No Hit
AATATACATTTACACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGG5220.10121261240998475No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5210.10101871851647905No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGAAAGT5160.10004924904895045No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5160.10004924904895045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)