FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515746
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTAGA39430.7645236220930458No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16070.3115874868636887No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14710.2852179173469111No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC13460.26098118065869635No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13020.25244984934444475No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12090.23441771724841295No Hit
CATTTACACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGGCCCACT11980.23228488441985007No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA11710.22704974929519567No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11600.2249169164666328No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11480.22259018974456418No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10720.2078542538381296No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9840.19079159120962644No Hit
GTGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGGAT9270.17973963927980052No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9260.1795457453862948No Hit
CACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT9230.17896406370577764No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8940.17334114079411184No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8550.16577927894738884No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8460.16403423390583738No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8450.16384034001233166No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8400.16287087054480306No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8110.15724794763313724No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA8090.1568601598461258No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7650.1483288285318742No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT7590.1471654651708399No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7580.1469715712773342No Hit
CAATAACATAGCCCATGAAATGTAACGAGTAACCCCATCTTTTTGTTTTG7440.14425705676825415No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7430.1440631628747484No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7270.14096086057865692No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCCTGTATGCATGTATT7040.13650130102802543No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7000.13572572545400255No Hit
CTCTATGACCGTTTCTCTCCCAAAAGTGAGACAAGAAATGTGAAACCACA6920.1341745743059568No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC6870.1332051048384282No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6810.13204174147739392No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTTCAAACC6810.13204174147739392No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGAA6690.1297150147553253No Hit
CATGCATACAGGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6560.12719439413975098No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6370.12351041016314232No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6340.12292872848262516No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT6310.12234704680210802No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6250.1211836834410737No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6240.12098978954756799No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACACC6230.12079589565406226No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC6220.12060200176055656No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6210.12040810786705083No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6150.11924474450601653No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6150.11924474450601653No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6060.11749969946446506No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6010.11653022999693646No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA6010.11653022999693646No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5920.114785184955385No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5850.11342792770084498No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5840.11323403380733928No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5760.11168288265929352No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5740.11129509487228209No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5730.11110120097877636No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCGTTACATTTCAT5680.11013173151124779No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT5630.10916226204371919No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT5570.10799889868268488No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5560.10780500478917918No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT5510.10683553532165058No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5450.10567217196061626No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5410.10489659638659339No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5380.10431491470607623No Hit
AATATACATTTACACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGG5360.1039271269190648No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5330.10334544523854766No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGAAAGT5320.10315155134504193No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5190.10063093072946759No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5170.10024314294245618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)