FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531855
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39170.7364789275272396No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA31240.5873781387784265No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15640.29406511173158095No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC15290.2874843707401454No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT14990.2818437356046291No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAGTTCGTAGGCCCACT14040.26398172434216094No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11900.2237451937088116No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA11520.21660038920382432No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTTTAAATTTTAT11470.21566028334790496No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11130.20926756352765324No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC10890.2047550554192402No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10680.20080661082437884No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10590.19911442028372395No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT10470.19685816622951746No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT10340.19441389100412707No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10200.1917815946075528No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT9840.1850128324449333No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9800.1842607477601978No Hit
TTCTAGAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9710.1825685572195429No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9020.16959509640785553No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8990.1690310328943039No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT8800.16545863064181027No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT8140.1530492333436745No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC7910.14872474640644537No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7880.14816068289289372No Hit
TTTTTCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC7840.14740859820815824No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC7750.14571640766750338No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT7360.13838358199133222No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT7240.1361273279371257No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA6930.13029867163042558No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6900.12973460811687398No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6890.12954658694569007No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6890.12954658694569007No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6670.12541012117964484No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCATCAAACTCTGCAAG6580.12371793063898995No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6540.12296584595425444No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6470.12164969775596733No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAT6460.12146167658478345No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6420.12070959190004794No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6410.12052157072886407No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA6370.11976948604412857No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT6350.1193934437017608No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6260.11770125316110595No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG6180.11619708379163494No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6080.11431687207979618No Hit
GTTCGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA6030.11337676622387681No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGGACAACAGAGT6020.11318874505269294No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6010.11300072388150907No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA5930.11149655451203806No Hit
CACCAAGACATTATTAAAAAATGTGAACAGTTCGTAGGCCCACTCACAGT5880.11055644865611867No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCA5850.10999238514256704No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5770.10848821577309606No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC5710.10736008874599279No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5700.10717206757480892No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5690.10698404640362505No Hit
GTTCTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5680.10679602523244118No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT5610.10547987703415404No Hit
AATATACATTTACACCAAGACATTATTAAAAAATGTGAACAGTTCGTAGG5570.10472779234941855No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5560.10453977117823465No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5540.10416372883586691No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5530.10397570766468305No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT5500.10341164415113141No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5480.10303560180876367No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC5480.10303560180876367No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTAGAA5370.10096736892574104No Hit
GTTATGGATCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT5350.10059132658337329No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5350.10059132658337329No Hit
CTCCGAAAGACACCAAATATTCTAGAACTGTTTCTCTTCCAAAAGTGAGA5340.10040330541218942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)