FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531855
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39210.7372310122119751No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA27650.5198785383234152No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14520.27300674055898694No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT14330.2694343383064933No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAGTTCGTAGGCCCACT14170.2664259995675513No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11890.22355717253762772No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11730.22054883379868576No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTTTAAATTTTAT11120.2090795423564693No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10920.2053191189327918No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10600.1993024414549078No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10550.19836233559898847No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC10520.1977982720854368No Hit
TTCTAGAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10520.1977982720854368No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT10160.1910295099228173No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10130.1904654464092657No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT10050.18896127703979468No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10010.18820919235505917No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9750.1833206419042784No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT9710.1825685572195429No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT9600.1805003243365203No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT8630.1622622707316844No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT8440.15868986847919075No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT7840.14740859820815824No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC7820.14703255586579048No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT7730.1453403653251356No Hit
TTTTTCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC7730.1453403653251356No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC7730.1453403653251356No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7710.14496432298276787No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7680.14440025946921622No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7450.1400757725319871No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC7270.13669139145067735No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCATCAAACTCTGCAAG7060.13274294685581597No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6940.13048669280160946No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6920.13011065045924172No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA6840.1286064810897707No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6770.1272903328914836No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA6600.12409397298135769No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6560.12334188829662221No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6520.1225898036118867No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6440.1210856342424157No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAT6390.12014552838649631No Hit
GTTCGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA6340.11920542253057693No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6270.11788927433228982No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG6210.11676114730518658No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6210.11676114730518658No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6200.11657312613400268No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGGACAACAGAGT6070.11412885090861231No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5990.1126246815391413No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5960.1120606180255897No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA5950.1118725968544058No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5890.11074446982730256No Hit
AATATACATTTACACCAAGACATTATTAAAAAATGTGAACAGTTCGTAGG5800.10905227928664767No Hit
CACCAAGACATTATTAAAAAATGTGAACAGTTCGTAGGCCCACTCACAGT5690.10698404640362505No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT5680.10679602523244118No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5650.10623196171888954No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA5650.10623196171888954No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5610.10547987703415404No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5560.10453977117823465No Hit
GTTCTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5500.10341164415113141No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5500.10341164415113141No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5470.1028475806375798No Hit
CTCCGAAAGACACCAAATATTCTAGAACTGTTTCTCTTCCAAAAGTGAGA5450.10247153829521204No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT5410.10171945361047653No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5350.10059132658337329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)