FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331919
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC43621.3141760489758043No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC15310.46125711393442376No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA14860.4476995893576445No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14170.42691138500658293No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12380.3729825650233943No Hit
CTATATATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC11850.3570148138551876No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10880.32779081643413No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT10310.31061795197020964No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10100.3042911071677126No Hit
TCCTAGTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGT9620.28982974761914804No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC9450.28470801611236474No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAAGCTCCTCC9300.28018884125343835No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT9290.27988756292950995No Hit
GATATATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC9060.2729581614791561No Hit
GCCTAATCATCTCGTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8390.25277251377595134No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8150.2455418340016691No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7650.23047791780524768No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7550.22746513456596337No Hit
CCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG7440.22415107300275067No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6810.20517053859525966No Hit
GTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTA6800.20486926027133123No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6410.19311940563812255No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6120.18438233424419814No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6070.182875942624556No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6050.18227338597669912No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAAGCT6040.1819721076527707No Hit
ATATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG6020.18136955100491384No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5980.18016443770920013No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5970.1798631593852717No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5900.17775421111777273No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5890.17745293279384428No Hit
AATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTTGTCT5600.16871586139991984No Hit
CTATACATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5450.16419668654099343No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5430.16359412989313657No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5420.16329285156920814No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC5420.16329285156920814No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC5310.15997879000599544No Hit
AATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT5290.15937623335813858No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5230.157568563414568No Hit
CTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTGTATGCCTGTATT5060.1524468319077847No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4960.14943404866850044No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4950.14913277034457204No Hit
GGATATATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC4890.14732510040100144No Hit
GTACAGACTTGGCCCCCAATACCACATCATCAATATAACTGAAAGCCAAA4820.14521615213350245No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4780.14401103883778874No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT4740.14280592554207502No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGCTTA4690.14129953392243289No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4670.14069697727457603No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4650.14009442062671917No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4610.13888930733100546No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4600.13858802900707703No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGT4560.13738291571136332No Hit
GCTGTAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGAC4540.13678035906350647No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGA4510.13587652409172118No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC4480.1349726891199359No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4480.1349726891199359No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCGCCTGA4470.13467141079600747No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4440.1337675758242222No Hit
ATATTAGTGTTGACATAACTAACTACTAGGTCCCTAGATGCTGGATCTTC4380.1319599058806516No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4380.1319599058806516No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4370.13165862755672317No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4360.13135734923279474No Hit
CCTCTATATATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4320.13015223593708103No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA4230.12744073102172518No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4200.1265368960499399No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4160.12533178275422618No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4140.12472922610636933No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4070.12262027783887033No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC4000.12051132957137134No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA3990.1202100512474429No Hit
GTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3920.1181011029799439No Hit
CATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATT3900.11749854633208705No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG3850.1159921547124449No Hit
CCTACAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3820.11508831974065961No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3820.11508831974065961No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3820.11508831974065961No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG3820.11508831974065961No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3740.11267809314923219No Hit
TCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCGCCTGAGC3700.11147297985351849No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3630.10936403158601948No Hit
CTCCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3620.10906275326209106No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG3590.10815891829030577No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3560.10725508331852049No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3550.10695380499459205No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3550.10695380499459205No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3500.10544741337494992No Hit
ACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG3470.10454357840316462No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3470.10454357840316462No Hit
ATGTGGTTATCCTGCGTTAATGCCCTTGTATGCCTGTATTCAATCTCGGC3450.10394102175530777No Hit
CATCAATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACT3450.10394102175530777No Hit
CATTTAAATCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT3380.10183207348780877No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3370.10153079516388033No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG3360.10122951683995192No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC3340.10062696019209505No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT3330.10032568186816664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)