FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331919
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC38711.166248391926946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14160.4266101066826545No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA12950.3901554294873147No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12540.3778030182062491No Hit
CTATATATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC11810.3558097005594738No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11610.34978413408090525No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11340.34164961933483773No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10360.31212434358985175No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8800.2651249250570169No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8560.25789424528273464No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8490.2557852970152356No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8320.25066356550845237No Hit
TCCTAGTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGT8160.2458431123255975No Hit
GATATATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC8150.2455418340016691No Hit
GCCTAATCATCTCGTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7950.23951626752310048No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAAGCTCCTCC7540.22716385624203497No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7500.22595874294632123No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6570.19793985882097742No Hit
CCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6380.19221557066633727No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6360.1916130140184804No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6210.18709383915955397No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6120.18438233424419814No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6060.18257466430062758No Hit
GTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTA6030.1816708293288423No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5990.18046571603312855No Hit
CTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTGTATGCCTGTATT5640.16992097469563358No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5640.16992097469563358No Hit
AATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTTGTCT5590.16841458307599144No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5570.16781202642813456No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC5540.16690819145634928No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5530.16660691313242085No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGCTTA5510.166004356484564No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5430.16359412989313657No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAAGCT5320.16028006832992386No Hit
GTACAGACTTGGCCCCCAATACCACATCATCAATATAACTGAAAGCCAAA5180.15606217179492587No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5150.1551583368231406No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5020.15124171861207103No Hit
ATATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4930.14853021369671515No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4930.14853021369671515No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4880.14702382207707304No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC4780.14401103883778874No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT4750.14310720386600345No Hit
CTATACATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4740.14280592554207502No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCGCCTGA4740.14280592554207502No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4730.1425046472181466No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4730.1425046472181466No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4670.14069697727457603No Hit
CTGCCTAATCATCTCGTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT4660.1403956989506476No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4630.13949186397886232No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4590.1382867506831486No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4560.13738291571136332No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4510.13587652409172118No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4490.1352739674438643No Hit
AATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT4480.1349726891199359No Hit
CCTCTATATATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4480.1349726891199359No Hit
GGATATATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC4420.1331650191763653No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4400.13256246252850845No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4310.12985095761315263No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGA4300.12954967928922417No Hit
GCTGTAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGAC4300.12954967928922417No Hit
ATATTAGTGTTGACATAACTAACTACTAGGTCCCTAGATGCTGGATCTTC4250.12804328766958203No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4230.12744073102172518No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT4190.12623561772601147No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4080.12292155616279875No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4080.12292155616279875No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4020.12111388621922818No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4010.12081260789529975No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4010.12081260789529975No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG3950.11900493795172919No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC3890.11719726800815862No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA3860.11629343303637334No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA3840.11569087638851647No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3770.11358192812101749No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG3730.11237681482530378No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3650.10996658823387634No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3590.10815891829030577No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG3560.10725508331852049No Hit
CATTTAAATCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT3510.10574869169887834No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3490.1051461350510215No Hit
CCTACAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3470.10454357840316462No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC3460.10424230007923621No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC3440.10363974343137934No Hit
ACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG3420.10303718678352249No Hit
TCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCGCCTGAGC3410.10273590845959406No Hit
CAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC3400.10243463013566562No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3400.10243463013566562No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT3380.10183207348780877No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG3340.10062696019209505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)