FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446939
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA18900.4228764999250457No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16370.3662692224218517No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC15930.35642447850825276No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14980.33516878142207324No Hit
GTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCATGTTCAGG14850.3322601070839645No Hit
ATTTCCGAGAGAGGACAACAGAGTTGTCAGTCCCGATAAGTTTCGCTCCA14610.3268902467674559No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA13160.29444734068855033No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTCGTTT12730.2848263409548059No Hit
CCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA12410.2776665271994612No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11570.2588720160916814No Hit
GACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACAC11260.2519359465161913No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11150.24947476053779152No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAA10900.2438811560414285No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10880.24343366768171942No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10770.2409724817033197No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10330.23112773778972076No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA10230.22889029599117552No Hit
CTCGAAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGG9960.22284920313510345No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9930.2221779705955399No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9830.21994052879699466No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9660.2161368777394678No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9640.21568938937975876No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9590.21457066848048614No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9490.21233322668194093No Hit
CTCAACAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9360.20942455234383214No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8920.1995798084302332No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8900.19913232007052417No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8890.19890857589066965No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8760.19599990155256086No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8450.1890638319770707No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG7990.1787715997037627No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTCGT7980.17854785552390817No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7900.176757902085072No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7700.17228301848798158No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7480.1673606465311821No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG7280.16288576293409165No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7160.1602008327758374No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTGGATGTTCCTGG7160.1602008327758374No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6980.15617343753845603No Hit
GATCATTAACCTAGTCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACAC6840.15304101902049275No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6810.15236978648092916No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6700.14990860050252944No Hit
GGAACATATTGTACTAAAAATCAAACAATGTTTTCGAAAACTGCCTGTAA6640.1485661354234023No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTAATTTCAT6580.14722367034427516No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6470.14476248436587544No Hit
ATTTTCGAGAGAGGACAACAGAGTTGTCAGTCCCGATAAGTTTCGCTCCA6300.14095883330834857No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT6240.13961636822922144No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6230.13939262404936692No Hit
GTCCCGTGCTGGTAGTGGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6140.13737892643067623No Hit
ATATTTTCCTGCTGGTGGCTCAAGTTCCGGAACAGTAAACCCTGTTCCGA6140.13737892643067623No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6060.13558897299184006No Hit
GGGTTTACTGTTCCGGAACTTGAGCCACCAGCAGGAAAATATAGGCCCCT6030.1349177404522765No Hit
GTGTGAACCCTGGCCCGAATGCTCCCGCTCCTACCTGATTTGCCTCTGGC6000.13424650791271292No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5940.13290404283358578No Hit
CTACTGTACCTGTCTTTAATCATGAGTGGCAAACTCCCTCCTTTCCTAAC5940.13290404283358578No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5930.13268029865373127No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5890.1317853219343132No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5870.13133783357460416No Hit
GTGGATATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG5800.1297716243156225No Hit
ATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC5780.12932413595591344No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATATCCACAT5720.12798167087678633No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5720.12798167087678633No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCAGGCGCCGATGGCC5690.12731043833722275No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG5680.12708669415736823No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5680.12708669415736823No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG5630.12596797325809564No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5610.12552048489838658No Hit
GTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5610.12552048489838658No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5570.1246255081789685No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5560.12440176399911397No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG5530.12373053145955042No Hit
CATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAAGGGGCAGCA5510.12328304309984137No Hit
GTTGTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC5480.1226118105602778No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5460.12216432220056876No Hit
CCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA5370.12015062458187807No Hit
ATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCT5370.12015062458187807No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5340.1194793920423145No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5340.1194793920423145No Hit
AATTAAGGGAGTAACCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA5290.1183606711430419No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5250.11746569442362381No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5240.11724195024376928No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5220.11679446188406024No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5220.11679446188406024No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5220.11679446188406024No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5210.11657071770420573No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCCTGCGTGGAACCTTTGTGGC5210.11657071770420573No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5200.1163469735243512No Hit
GGGTTCACACCACCCCACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5190.11612322934449668No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5140.11500450844522407No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5120.11455702008551502No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5110.1143332759056605No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5110.1143332759056605No Hit
CTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCC5070.11343829918624242No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5050.11299081082653338No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5010.1120958341071153No Hit
CTTTTACACAATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATA5010.1120958341071153No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5010.1120958341071153No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC4970.11120085738769721No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4960.11097711320784268No Hit
CCCCAAAAGACCGCCGTGGGGTGGTGTGAACCCTGGCCCGAATGCTCCCG4910.10985839230857007No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCAAGTTCCGGA4900.10963464812871555No Hit
CAACAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAAT4890.10941090394886104No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4880.1091871597690065No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCCACTACC4880.1091871597690065No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4840.10829218304958842No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4830.1080684388697339No Hit
CTCGGAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGG4810.10762095051002486No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4790.10717346215031581No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG4770.10672597379060678No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4750.10627848543089773No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC4720.10560725289133416No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC4670.10448853199206155No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4650.10404104363235252No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTAGTACAATA4620.10336981109278895No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC4610.10314606691293443No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4610.10314606691293443No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG4600.1029223227330799No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4590.10269857855322538No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4580.10247483437337086No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4580.10247483437337086No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCAC4580.10247483437337086No Hit
TCCACAGAGTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCA4580.10247483437337086No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACGAGGCATTATT4570.10225109019351634No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTG4560.10202734601366181No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4540.10157985765395278No Hit
GAATATGGTGACCCACAAAATGAGGCGCTGCGTGTAGTTTCTCTCTTATA4490.10046113675468017No Hit
GGTGTGAACCCTGGCCCGAATGCTCCCGCTCCTACCTGATTTGCCTCTGG4490.10046113675468017No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4470.10001364839497112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)