FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400635
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA18600.464262982515257No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT18000.4492867572728294No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17430.4350593432925231No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA16970.4235775706066619No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15850.39562195015413026No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC11590.289290750932894No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11050.2758121482147091No Hit
ATGTAAGACCTTGGGCAAGACCTGGCTGGCGTTCACGGTGGTCTCCATGC10640.2655783942990503No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10380.25908869669399825No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCCGG9970.2488549427783394No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9160.22863703870106208No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9040.22564179365257656No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8890.22189773734196963No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8800.21965130355560547No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8680.21665605850711994No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7900.197186965691964No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7890.19693736193792355No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACT7530.18795162679246696No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7450.1859547967601433No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7250.18096272167933405No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7190.1794650991550913No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7150.17846668413892944No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7120.17771787287680807No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTCTTTTCG7050.17597064659852485No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6870.17147777902579653No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6740.16823293022327057No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6700.1672345152071087No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6680.1667353076990278No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6660.16623610019094687No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6560.16374006265054225No Hit
GTCCCGTGCTGGTAGTTGTTGTTCCTGGAAGTAGAGGACAAACGGGCAAC6430.16049521384801627No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6290.15700076129144983No Hit
TCCTCAGGCCATGCAGTGGAATTCCAGCACTTTCCACCAAGCTCTGCTAG5920.14776542239195278No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5890.1470166111298314No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5840.14576859235962908No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5840.14576859235962908No Hit
GTCTTACATAAGCGGACTATTGGACTCTCAGCAATGTCAACGACCGACCT5770.14402136608134586No Hit
GTCCAATAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGCTGGCGTTC5760.1437717623273054No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5760.1437717623273054No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAAAGTGCTGG5670.14152532854094124No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5650.14102612103286033No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTTC5540.13828047973841526No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5450.1360340459520511No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5400.13478602718184882No Hit
CTGTAAGAGGGCCCACATATTGTTGGCATCTATTGATAATATCCTCTTTC5320.13278919714952514No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5290.13204038588740374No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCAC5280.1317907821333633No Hit
ACTTCTCGGCACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT5260.13129157462528238No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC5160.12879553708487776No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5140.12829632957679682No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAA5120.12779712206871593No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5040.12580029203639223No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5020.1253010845283113No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4990.12455227326618992No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4940.12330425449598763No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4870.1215570282177044No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACTC4820.12030900944750209No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4790.11956019818538072No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4770.11906099067729979No Hit
ATATAAGAGAGAAACCACTCGTAGCGCCTCATTTTGTGGGTCACCATATT4750.11856178316921885No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4730.11806257566113795No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4720.11781297190709748No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG4630.11556653812073335No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4620.11531693436669288No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4610.11506733061265241No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4580.11431851935053104No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA4540.11332010433436918No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTCTTTTCGTTAAC4530.11307050058032872No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4530.11307050058032872No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4490.11207208556416688No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4460.11132327430204551No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4380.10932644426972181No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4350.10857763300760044No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4340.10832802925355997No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA4300.10732961423739813No Hit
TCCTAATGTGGTGCTCTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGA4290.10708001048335768No Hit
CTCTTACAGTTAACGAAAAGAGGAGATTAAAATTGATTATGCCTGCTAGG4290.10708001048335768No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4280.1068304067293172No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCCGACGACCCGTCTCG4270.10658080297527674No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4250.10608159546719584No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4250.10608159546719584No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAATAGTCCGCTTATGT4120.10283674666466984No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCCGACGACCCGTCTCGGGGCCG4100.10233753915658891No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4090.10208793540254846No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4090.10208793540254846No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4040.10083991663234614No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCAGCCAGGTCTTGCCC4030.1005903128783057No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4030.1005903128783057No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4030.1005903128783057No Hit
CTACTGTACCTGTCTTCAATCCTGACTGGCAAACTCCCTCTTTTCCTCGC4020.10034070912426524No Hit
GCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4020.10034070912426524No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4010.10009110537022477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)