FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542879
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT19830.36527476656860924No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT18080.33303922236815203No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17450.32143442645598747No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16290.30006686572882724No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT14490.26691030597978554No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13830.2547529007384703No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC13170.24259549549715498No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12930.23817462086394942No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG12860.23688519909593114No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT12270.22601721562263413No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11370.20943893574811331No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10070.18549253148491654No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG9690.1784928133156744No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9660.1779402039865237No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG9540.1757297666699209No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9400.17315092313388436No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA9110.1678090329520943No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC9040.16651961118407602No Hit
CATTTAAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT8980.1654143925257746No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8910.16412497075775634No Hit
ATGTAAGACCTTAGGCAACACCTGGCGGGCGTTCACGGTGGTCTCCATGC8770.16154612722171977No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8770.16154612722171977No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8710.1604409085634184No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8670.15970409612455078No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8600.15841467435653248No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG8420.15509901838162832No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8340.15362539350389312No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC8180.15067814374842275No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8160.15030973752898896No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCACTAT8090.1490203157609707No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8050.14828350332210308No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7950.14644147222493412No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7560.13925755094597508No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7190.13244203588644984No Hit
ATTTATAGGAAGTTTCCGAAAACAGTGTTTGATTTTTTGTACAATATGTT7140.13152102033786534No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7100.13078420789899775No Hit
GGATATTATCAATAGATGTCAAGAATATGTGGGCCCTCTTACAGTTAACG7100.13078420789899775No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6770.12470550527834011No Hit
ATATTATCATTGACATAGCTTACTACTAATTCCCTGGATGCCGGGTCTTC6750.12433709905890632No Hit
ATATAAGAGAGAAACTACTCGCAGCGCTTCATTTTGTGGGTCACCATATT6670.12286347418117112No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6580.12120564619371905No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6580.12120564619371905No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6580.12120564619371905No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6540.12046883375485146No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6500.11973202131598384No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6480.11936361509655007No Hit
CCTCGATACCGCATCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAAC6440.11862680265768245No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6430.11844259954796557No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6380.11752158399938106No Hit
GATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCG6380.11752158399938106No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6360.11715317777994728No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6340.11678477156051349No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6330.11660056845079658No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6310.11623216223136279No Hit
CTACTGTACCTGTCTTTAATCCCGAATGGCAAACTCCCTCTTTTCCTCAT6260.11531114668277831No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC6220.11457433424391071No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6180.11383752180504311No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGTGGTAAAAA6180.11383752180504311No Hit
GGAACATATTGTACAAAAAATCAAACACTGTTTTCGGAAACTTCCTATAA6130.11291650625645862No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6090.11217969381759102No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCTAAATTACTTCCCACCC6070.11181128759815723No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCT6020.11089027204957275No Hit
AGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTTAAATGTATA6000.11052186583013894No Hit
CTGTAAGAGGGCCCACATATTCTTGACATCTATTGATAATATCCTCTTTC5950.10960085028155445No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5940.10941664717183755No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAACACCTGGCGGGCGTTC5870.10812722540381928No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5780.10646939741636718No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5770.10628519430665027No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5760.10610099119693339No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5750.1059167880872165No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5740.10573258497749959No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCTTCAT5620.1035221476608968No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5510.10149591345401093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)