FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542879
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17870.32917095706409716No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17390.32032920779768603No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT17220.3171977549324988No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14860.27372582103931076No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12850.23670099598621422No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT12800.23577998043762977No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG12710.23412215245017767No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12170.22417518452546517No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT11180.2059390766634922No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10650.19617631184849663No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10050.18512412526548275No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG9500.17499295423105332No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9210.16965106404926328No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC9080.1672564236229436No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG8910.16412497075775634No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8860.16320395520917183No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8760.16136192411200287No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8490.1563884401496466No Hit
CATTTAAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT8460.1558358308204959No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8460.1558358308204959No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8400.15473061216219453No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG8150.15012553441927207No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8020.1477308939929524No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7710.14202059759172855No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7680.14146798826257787No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7650.14091537893342715No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7590.13981016027512577No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7540.1388891447265413No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7520.13852073850710747No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7490.13796812917795678No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7320.13483667631276952No Hit
ATGTAAGACCTTAGGCAACACCTGGCGGGCGTTCACGGTGGTCTCCATGC7310.13465247320305263No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7270.133915660764185No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCACTAT7190.13244203588644984No Hit
ATTTATAGGAAGTTTCCGAAAACAGTGTTTGATTTTTTGTACAATATGTT7000.12894217680182876No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6720.12378448972975563No Hit
ATATTATCATTGACATAGCTTACTACTAATTCCCTGGATGCCGGGTCTTC6700.12341608351032181No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6670.12286347418117112No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6600.12157405241315285No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6520.12010042753541766No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6520.12010042753541766No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6400.11788999021881488No Hit
ATATAAGAGAGAAACTACTCGCAGCGCTTCATTTTGTGGGTCACCATATT6390.11770578710909797No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6370.11733738088966418No Hit
CCTCGATACCGCATCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAAC6360.11715317777994728No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCTAAATTACTTCCCACCC6270.11549534979249519No Hit
GTCTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG6210.1143901311341938No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGTGGTAAAAA6210.1143901311341938No Hit
GGATATTATCAATAGATGTCAAGAATATGTGGGCCCTCTTACAGTTAACG6070.11181128759815723No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6040.11125867826900654No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5990.11033766272042206No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG5960.10978505339127136No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5940.10941664717183755No Hit
GGAACATATTGTACAAAAAATCAAACACTGTTTTCGGAAACTTCCTATAA5730.1055483818677827No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5620.1035221476608968No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5620.1035221476608968No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5600.10315374144146301No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5560.10241692900259541No Hit
GTCCCAGACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG5550.10223272589287853No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5540.10204852278316162No Hit
GATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCG5540.10204852278316162No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC5530.10186431967344474No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCAAATTTTGGCCAGGACACG5530.10186431967344474No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCT5490.10112750723457714No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5450.10039069479570953No Hit
AGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTTAAATGTATA5440.10020649168599265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)