FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444886
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA20690.4650629599492904No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19130.4299977971884933No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17710.39807950800879327No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17100.38436813026258415No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA16170.3634638986167243No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC15290.3436835503926849No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12380.27827353524273635No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA12140.2728788948179983No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC11590.2605161771779737No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGAGAATATCCTCTTTC10880.24455703258812372No Hit
GCATTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC10400.23376775173864764No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT10300.23151998489500683No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC10240.2301713247888223No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT10010.22500146104844834No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG9460.21263874340842373No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9120.20499633614004487No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8770.19712915218730193No Hit
GTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGACA8750.19667959881857375No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8330.18723897807528223No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8300.18656464802218994No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8130.18274344438800053No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG8110.18229389101927235No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT7940.17847268738508293No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7940.17847268738508293No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG7860.17667447391017024No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7650.1719541635385245No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7550.16970639669488363No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7480.16813295990433505No Hit
CTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC7460.16768340653560687No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7330.16476130963887378No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7240.16273831947959702No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG7040.15824278579231532No Hit
TCCACAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATG7000.157343679054859No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6990.15711890237049492No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGAGAAAGGCATTAAACCTTAT6960.15644457231740266No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6950.15621979563303856No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG6830.15352247542066955No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6810.1530729220519414No Hit
GTCCCGTGCTGGTAGTTGTTGTTCCTGGAAGTAGAGGACAAACGGGCAAC6700.15060037852393648No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6530.14677917488974704No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCACGCGCTGATGGCC6440.14475618473047028No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6350.14273319457119352No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6240.1402606510431886No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6230.1400358743588245No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6120.1375633308308196No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6040.1357651173559069No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6040.1357651173559069No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5910.13284302045917382No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5840.1312695836686252No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5760.12947137019371255No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5700.12812271008752804No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5650.12699882666570764No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5640.12677404998134353No Hit
ATTTACACACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT5530.12430150645333861No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCATTAAGGCA5500.12362717640024634No Hit
TTATTTACACACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTA5390.12115463287224143No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCGTGCGTGGAACCTTTGTGGC5260.11823253597550833No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5240.11778298260678016No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA5210.11710865255368792No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5190.11665909918495973No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5170.11620954581623158No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5160.11598476913186749No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5100.11463610902568298No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5080.11418655565695482No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5070.11396177897259074No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5060.11373700228822665No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA5060.11373700228822665No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5050.11351222560386255No Hit
CTCTCACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5020.11283789555077031No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT4940.11103968207585764No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4920.11059012870712948No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG4900.1101405753384013No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAACTTAGACAAATATT4850.10901669191658088No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4810.10811758517912454No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4790.10766803181039637No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4780.1074432551260323No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4770.10721847844166821No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4710.1058698183354837No Hit
GGATATATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGC4670.10497071159802736No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4670.10497071159802736No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG4530.10182383801693018No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC4520.10159906133256609No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4510.10137428464820201No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4480.10069995459510976No Hit
GTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT4470.10047517791074569No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC4460.10025040122638158No Hit
GTACCTTACCACAGGAACATATTGTACACAAAATCAAACAATGTTTTCGG4450.1000256245420175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)