FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444886
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19660.4419109614597897No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16640.37402840278183624No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA16210.3643630053541806No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14100.3169351249533588No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA14100.3169351249533588No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC11520.2589427403874251No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10970.24658002274740046No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGAGAATATCCTCTTTC10610.23848806211029341No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA10520.23646507195101665No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC10170.22859788799827369No Hit
GCATTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC9750.2191572672549822No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT9600.21578561698952092No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT9370.21061575324914697No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG9090.2043220060869526No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8530.1917345117625639No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG8490.19083540502510757No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8480.19061062834074347No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8260.18566554128473362No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8180.18386732780982096No Hit
GTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGACA8180.18386732780982096No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG8010.1800461241756315No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7980.17937179412253926No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7890.1773488039632625No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7820.17577536717271391No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT7480.16813295990433505No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7050.1584675624766794No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG7040.15824278579231532No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7030.15801800910795125No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6990.15711890237049492No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG6950.15621979563303856No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCACGCGCTGATGGCC6940.15599501894867449No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6890.15487113552685405No Hit
CTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC6880.15464635884248998No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6840.15374725210503365No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6610.14857738836465972No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6570.14767828162720337No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6570.14767828162720337No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6560.1474535049428393No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6540.14700395157411114No Hit
TCCACAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATG6520.14655439820538294No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6460.14520573809919843No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6440.14475618473047028No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6410.14408185467737802No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6380.14340752462428577No Hit
GTCCCGTGCTGGTAGTTGTTGTTCCTGGAAGTAGAGGACAAACGGGCAAC6300.14160931114937308No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6190.13913676762136817No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCGTGCGTGGAACCTTTGTGGC6120.1375633308308196No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5890.13239346709044564No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGAGAAAGGCATTAAACCTTAT5870.13194391372171746No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5630.12654927329697946No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5620.12632449661261536No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5510.12385195308461044No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5500.12362717640024634No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5350.12025552613478509No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5350.12025552613478509No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5280.11868208934423652No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5270.11845731265987242No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5100.11463610902568298No Hit
ATTTACACACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT5070.11396177897259074No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5050.11351222560386255No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5030.11306267223513439No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5020.11283789555077031No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5010.11261311886640622No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4990.11216356549767807No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4940.11103968207585764No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC4920.11059012870712948No Hit
GGATATATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGC4920.11059012870712948No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4840.1087919152322168No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAACTTAGACAAATATT4830.1085671385478527No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCATTAAGGCA4740.10654414838857594No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT4700.10564504165111963No Hit
GTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG4660.10474593491366327No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4580.1029477214387506No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4560.10249816807002243No Hit
CCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTT4530.10182383801693018No Hit
CTCTCACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG4520.10159906133256609No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4520.10159906133256609No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4520.10159906133256609No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4500.10114950796383793No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4480.10069995459510976No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4470.10047517791074569No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4460.10025040122638158No Hit
TTATTTACACACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTA4460.10025040122638158No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC4460.10025040122638158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)