FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381040
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22450.5891769892924628No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20800.5458744488767584No Hit
GTGTAAAGGGAGCGGCAAATCCCAAAAGACCCACAATTCTTTGGCACACT18670.48997480579466723No Hit
CCCTTTACACAATGTGGTTATCCAGCATTAATGCCTTTGTATGCATGTAT16490.4327629645181608No Hit
GTATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAG13160.34537056477010286No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11180.2934075162712576No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9540.2503674154944363No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG9500.24931765693890404No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9320.24459374343900903No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8930.2343585975225698No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT8860.23252152005038842No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7960.2089019525509133No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7890.2070648750787319No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7480.19630484988452657No Hit
GTATACATGCATACAAAGGCATTAATGCTGGATAACCACATTGTGTAAAG7440.19525509132899435No Hit
GTGCCAAAGAATTGTGGGTCTTTTGGGATTTGCCGCTCCCTTTACACAAT7370.19341801385681293No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7120.18685702288473652No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7040.18475750577367206No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7010.18397018685702288No Hit
ATTTATAGGAAGTTTGCGAAAACATTGTTTGATTTTTTGTACAATATGTT6900.18108335082930924No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6870.18029603191266008No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC6830.17924627335712787No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6800.17845895444047868No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6610.1734726013017006No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6510.17084820491287003No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6330.16612429141297502No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6320.16586185177409196No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6320.16586185177409196No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6270.16454965357967669No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC6200.16271257610749526No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6150.16140037791307998No Hit
CCTATAAATAGGCCTATTGATTGGAAAGTGTGCCAAAGAATTGTGGGTCT6020.15798866260760025No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5970.15667646441318497No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5940.1558891454965358No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5930.15562670585765273No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG5920.1553642662187697No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAACG5860.15378962838547133No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5760.15116523199664078No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGAGTGACGATATGG5740.15064035271887466No Hit
GTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5720.15011547344110857No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5660.1485408356078102No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5600.14696619777451186No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC5430.1425047239134999No Hit
GGAAAGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5400.14171740499685073No Hit
GCACAAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT5380.1411925257190846No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5350.14040520680243543No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5340.1401427671635524No Hit
GTTTTGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG5330.1398803275246693No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5330.1398803275246693No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG5320.13961788788578627No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5320.13961788788578627No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5290.1388305689691371No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5200.1364686122191896No Hit
ATTGACAACAGTACCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5110.13410665546924208No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGCAAACTTCCTATAA5030.13200713835817762No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5030.13200713835817762No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5020.13174469871929456No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5000.13121981944152844No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC4940.1296451816082301No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4910.12885786269158092No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4910.12885786269158092No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4750.12465882846945202No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4740.12439638883056897No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4640.12177199244173839No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4630.12150955280285534No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4630.12150955280285534No Hit
GTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4540.11914759605290784No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC4520.11862271677514172No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT4500.11809783749737561No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4480.11757295821960949No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4470.11731051858072644No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4450.1167856393029603No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4400.11547344110854503No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4390.11521100146966198No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA4380.11494856183077894No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4370.11468612219189586No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4350.11416124291412975No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4320.11337392399748059No Hit
ACAGCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT4320.11337392399748059No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4320.11337392399748059No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4320.11337392399748059No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTGAC4300.11284904471971445No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4280.11232416544194836No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG4280.11232416544194836No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4270.1120617258030653No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4190.10996220869200085No Hit
AAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT4180.10969976905311778No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG4180.10969976905311778No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4160.10917488977535167No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4150.10891245013646862No Hit
CTCTTACAGTCAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG4100.10760025194205332No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC4100.10760025194205332No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG4080.10707537266428722No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4080.10707537266428722No Hit
CAATAGGCCTATTTATAGGAAGTTTGCGAAAACATTGTTTGATTTTTTGT4030.10576317446987192No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4010.10523829519210581No Hit
AAATAGGCCTATTGATTGGAAAGTGTGCCAAAGAATTGTGGGTCTTTTGG3980.10445097627545664No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC3920.10287633844215831No Hit
GGTTTGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGG3920.10287633844215831No Hit
GTTTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCGGGGCCG3920.10287633844215831No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT3900.10235145916439219No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC3900.10235145916439219No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3890.10208901952550914No Hit
ACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3880.10182657988662606No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3860.10130170060885996No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3840.10077682133109384No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA3840.10077682133109384No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3830.10051438169221079No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA3830.10051438169221079No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3820.10025194205332774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)