FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381040
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA18870.49522359857232834No Hit
GTGTAAAGGGAGCGGCAAATCCCAAAAGACCCACAATTCTTTGGCACACT17330.45480789418433765No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17090.44850934285114424No Hit
CCCTTTACACAATGTGGTTATCCAGCATTAATGCCTTTGTATGCATGTAT16290.4275141717404997No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11670.3062670585765274No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG9790.2569284064665127No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8800.23094688221709006No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8380.2199244173840017No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8150.21388830568969136No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8100.21257610749527608No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7650.20076632374553854No Hit
GTATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAG7610.1997165651900063No Hit
GTATACATGCATACAAAGGCATTAATGCTGGATAACCACATTGTGTAAAG7430.19499265169011126No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6690.17557211841276507No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6580.17268528238505143No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6530.17137308419063615No Hit
ATTTATAGGAAGTTTGCGAAAACATTGTTTGATTTTTTGTACAATATGTT6510.17084820491287003No Hit
GTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6310.1655994121352089No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6310.1655994121352089No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6190.16245013646861223No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6170.1619252571908461No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6150.16140037791307998No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6130.16087549863531386No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6100.1600881797186647No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6080.1595633004408986No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6040.15851354188536637No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6020.15798866260760025No Hit
GTGCCAAAGAATTGTGGGTCTTTTGGGATTTGCCGCTCCCTTTACACAAT5930.15562670585765273No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5930.15562670585765273No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5890.15457694730212052No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC5890.15457694730212052No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5870.15405206802435442No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGAGTGACGATATGG5820.15273986982993912No Hit
CCTATAAATAGGCCTATTGATTGGAAAGTGTGCCAAAGAATTGTGGGTCT5780.15169011127440687No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC5530.14512912030233047No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5420.14224228427461685No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5400.14171740499685073No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5400.14171740499685073No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5370.14093008608020155No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5360.1406676464413185No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA5270.138305689691371No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5210.13673105185807263No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5190.13620617258030654No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAACG5150.1351564140247743No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5110.13410665546924208No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5080.1333193365525929No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5080.1333193365525929No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5060.13279445727482678No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5040.13226957799706068No Hit
GTTTTGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG5040.13226957799706068No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4970.13043250052487929No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4900.1285954230526979No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG4890.12833298341381483No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4890.12833298341381483No Hit
ATTGACAACAGTACCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4840.12702078521939955No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC4840.12702078521939955No Hit
GTTTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCGGGGCCG4830.12675834558051646No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4830.12675834558051646No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4810.12623346630275037No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4750.12465882846945202No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4750.12465882846945202No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4690.1230841906361537No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4650.12203443208062147No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT4620.12124711316397228No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4600.12072223388620616No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGCAAACTTCCTATAA4500.11809783749737561No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4500.11809783749737561No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG4500.11809783749737561No Hit
GGAAAGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4470.11731051858072644No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTGAC4430.11626076002519421No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4390.11521100146966198No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4330.11363636363636363No Hit
GCACAAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT4320.11337392399748059No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4110.10786269158093639No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4060.10655049338652109No Hit
CAATAGGCCTATTTATAGGAAGTTTGCGAAAACATTGTTTGATTTTTTGT4030.10576317446987192No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG3990.10471341591433969No Hit
CTCTTACAGTCAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG3970.1041885366365736No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3960.10392609699769054No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3940.10340121771992442No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA3940.10340121771992442No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3920.10287633844215831No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3860.10130170060885996No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT3860.10130170060885996No Hit
GTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3860.10130170060885996No Hit
ACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3850.10103926096997691No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3820.10025194205332774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)