FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327424
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19380.5918930805316653No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT15140.46239738076622366No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT14790.4517078772478499No Hit
CCCTTTACACAGTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14470.4419346168881939No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14230.4346046716184519No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT13060.39887118842845976No Hit
CCCAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC9720.29686278342455047No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT9640.29441946833463645No Hit
CCCAGGGTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA9270.2831191360437842No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8990.2745675332290852No Hit
GCTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8250.25196686864738077No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT7870.2403611219702893No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7370.22509040265832683No Hit
CCCAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC7110.21714962861610634No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7090.21653879984362784No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6710.20493305316653634No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6490.19821393666927287No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6360.19424354964816265No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTGCCACAG6350.19393813526192338No Hit
GTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGC6320.1930218921032056No Hit
ACCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACGACAGAGT6320.1930218921032056No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACC5900.18019448788115713No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGGGTCCTCCCC5900.18019448788115713No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC5850.1786674159499609No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCG5810.1774457584050039No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5810.1774457584050039No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5650.1725591282251759No Hit
ATATGTTCCTGTGGCAAGGTGCCCCAACTTCCAATTACATATCCCATGAA5590.17072664190774042No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5430.16584001172791243No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5310.16217503909304146No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5270.16095338154808445No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5220.1594263096168882No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACACTGTGTAAAG5200.1588154808444097No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5120.1563721657544957No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA5010.15301260750586396No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4860.14843139171227523No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4690.14323934714620798No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4580.13987978889757624No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4540.13865813135261923No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4520.13804730258014072No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4510.13774188819390148No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4480.13682564503518374No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4450.135909401876466No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG4420.13499315871774825No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4420.13499315871774825No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4410.13468774433150899No Hit
GTAACCACACTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTC4380.13377150117279124No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4370.133466086786552No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4330.13224442924159502No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC4330.13224442924159502No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA4310.13163360046911649No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4280.13071735731039874No Hit
ACCAACACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTC4280.13071735731039874No Hit
AATACGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTGTCT4050.12369282642689602No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG4050.12369282642689602No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3980.12155492572322127No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3960.12094409695074276No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3870.11819536747458952No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG3860.11788995308835026No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA3810.11636288115715403No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT3790.11575205238467552No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3770.11514122361219704No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG3710.11330873729476154No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3680.11239249413604378No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC3660.11178166536356528No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3560.1087275215011728No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3550.10842210711493354No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3540.10811669272869429No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC3530.10781127834245505No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3530.10781127834245505No Hit
ATTGACAACAGTACCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3520.10750586395621578No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3500.1068950351837373No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3490.10658962079749805No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3490.10658962079749805No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3450.10536796325254104No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG3440.1050625488663018No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3420.10445172009382331No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3410.10414630570758404No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3410.10414630570758404No Hit
ATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGACTCTACCGTCCCCTT3410.10414630570758404No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3400.1038408913213448No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG3390.10353547693510556No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAACCTAGCAGCC3350.10231381939014857No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT3350.10231381939014857No Hit
ATGTTCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCA3340.1020084050039093No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3330.10170299061767005No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC3310.10109216184519154No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3300.1007867474589523No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG3300.1007867474589523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)