FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327424
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17530.5353914190774042No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT14480.4422400312744331No Hit
CCCTTTACACAGTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13390.40894986317435494No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT13310.406506548084441No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12360.37749218139171226No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT11850.3619160476935106No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8670.26479427286942925No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8420.257158913213448No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7730.2360853205629398No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT7570.23119869038311183No Hit
GCTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG7500.22906078967943705No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCG7260.2217308444096951No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7080.21623338545738857No Hit
CCCAGGGTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA7010.21409548475371387No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6660.2034059812353401No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTGCCACAG6650.20310056684910086No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6290.1921056489444879No Hit
GTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGC5900.18019448788115713No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGGGTCCTCCCC5840.17836200156372167No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5780.17652951524628616No Hit
ATATGTTCCTGTGGCAAGGTGCCCCAACTTCCAATTACATATCCCATGAA5530.16889415559030493No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACC5500.1679779124315872No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACACTGTGTAAAG5460.16675625488663018No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5390.16461835418295545No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5300.1618696247068022No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC5230.15973172400312743No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5190.15851006645817045No Hit
ACCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACGACAGAGT5140.1569829945269742No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5030.15362343627834246No Hit
CCCAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC5000.15270719311962472No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4970.15179094996090697No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4940.1508747068021892No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4780.1459880766223612No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4780.1459880766223612No Hit
CCCAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC4700.14354476153244722No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4620.14110144644253322No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG4580.13987978889757624No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4570.13957437451133697No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4460.13621481626270524No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4460.13621481626270524No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA4410.13468774433150899No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4390.13407691555903048No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4340.13254984362783426No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4260.13010652853792026No Hit
ACCAACACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTC4250.129801114151681No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4240.12949569976544176No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4200.12827404222048475No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4190.1279686278342455No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4150.1267469702892885No Hit
GTAACCACACTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTC4110.1255253127443315No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG3990.12186034010946052No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3960.12094409695074276No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC3930.12002785379202502No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3900.11911161063330726No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3900.11911161063330726No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG3890.11880619624706802No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG3860.11788995308835026No Hit
ATTGACAACAGTACCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3840.11727912431587179No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3750.11453039483971852No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3740.11422498045347929No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3730.11391956606724002No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC3730.11391956606724002No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA3730.11391956606724002No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3690.11269790852228302No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3640.11117083659108679No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3610.11025459343236904No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC3590.10964376465989054No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC3540.10811669272869429No Hit
CTGCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGT3530.10781127834245505No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3530.10781127834245505No Hit
AATACGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTGTCT3510.10720044956997654No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3480.10628420641125878No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG3480.10628420641125878No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3460.1056733776387803No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3430.10475713448006255No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT3400.1038408913213448No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA3320.10139757623143081No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC3320.10139757623143081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)