FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297557
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14260.4792359111027467No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA13300.4469731849696025No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10870.3653081594450811No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9630.3236354715231031No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8530.28666776449554204No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG7980.2681839109817615No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7650.25709359887349315No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA7640.25675752880960623No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7580.2547411084262847No Hit
GGCCCATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG7130.23961795555137336No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6690.2248308727403489No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6610.22214231222925357No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT6520.21911768165427128No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6350.21340449056819363No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6320.2123962803765329No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6250.21004378992932446No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC6210.2086995096737768No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCTTAT6210.2086995096737768No Hit
CTATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCAATC6190.20802736954600295No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5960.2002977580766038No Hit
GTTGTCAATATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG5520.18551067526557935No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5140.17274001283787643No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5140.17274001283787643No Hit
CATGTATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5100.17139573258232876No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5000.16803503194345956No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4910.1650104013684773No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCTTCCTGC4870.16366612111292964No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4870.16366612111292964No Hit
GTAGTAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4820.16198577079349502No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC4760.15996935041017352No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4700.157952930026852No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4470.15022331855745286No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4470.15022331855745286No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGTTGGAATGTTGTGG4420.14854296823801827No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4400.14787082811024443No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACCC4360.14652654785469676No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4300.14451012747137523No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4290.14417405740748832No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4290.14417405740748832No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA4260.14316584721582756No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4050.13610837587420224No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA4040.13577230581031532No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCAACAAGCTCTGCTAG4040.13577230581031532No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4020.1351001656825415No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC3950.13274767523533307No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA3870.13005911472423773No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3860.1297230446603508No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTCCT3850.1293869745964639No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3790.12737055421314236No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3780.12703448414925544No Hit
GGATTAAAGACAGGTACAGTAGTAGAATAAAGCCCAGTAAAGTTTCCCAC3780.12703448414925544No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3750.12602627395759466No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3710.12468199370204701No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3670.12333771344649933No Hit
GTCTTTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA3660.12300164338261241No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3640.12232950325483857No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3610.12132129306317782No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3580.12031308287151704No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3550.11930487267985629No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC3510.11796059242430862No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3510.11796059242430862No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC3500.1176245223604217No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT3480.11695238223264787No Hit
CCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATGT3470.11661631216876095No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG3470.11661631216876095No Hit
GTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3460.11628024210487403No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3430.11527203191321327No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3370.11325561152989176No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC3370.11325561152989176No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3360.11291954146600484No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC3350.11258347140211793No Hit
ATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAAATAGA3340.11224740133823098No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTA3340.11224740133823098No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAAC3310.11123919114657023No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC3310.11123919114657023No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3310.11123919114657023No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3250.10922277076324872No Hit
CTATTAATAATGTCTTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG3230.10855063063547488No Hit
ACATATTGTTGACATCTATTAATAATGTCTTCCTGCAAATGAATGTGAGG3230.10855063063547488No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3220.10821456057158796No Hit
TTCTACTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3210.10787849050770104No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3200.10754242044381412No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGGA3180.10687028031604029No Hit
GGCCTATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG3160.10619814018826645No Hit
GTATGGATTGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3150.10586207012437954No Hit
GGAACATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAA3150.10586207012437954No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG3140.10552600006049262No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT3130.1051899299966057No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT3120.10485385993271878No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3120.10485385993271878No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3110.10451778986883187No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3100.10418171980494495No Hit
GGCCTACATATTGTTGACATCTATTAATAATGTCTTCCTGCAAATGAATG3090.103845649741058No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT3080.10350957967717109No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3080.10350957967717109No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC3070.10317350961328417No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG3070.10317350961328417No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3060.10283743954939725No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3050.10250136948551034No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3040.10216529942162342No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3040.10216529942162342No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTG3030.1018292293577365No Hit
GTACAGTAGTAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA3020.10149315929384958No Hit
AAATAGACCTGTTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG3010.10115708922996267No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3010.10115708922996267No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG3000.10082101916607575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)