FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297557
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14260.4792359111027467No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA11560.3884969938532785No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9930.33371757343971076No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG8600.28902025494275047No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8490.2853234842399944No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8250.2772578027067083No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7810.2624707198956838No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA7470.25104433772352863No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7000.2352490447208434No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6940.2332326243375219No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6770.22751943325144425No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCTTAT6330.2127323504404198No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6300.21172414024875907No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT6180.207691299482116No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6110.20533880903490762No Hit
GGCCCATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG6090.20466666890713378No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC6080.20433059884324684No Hit
CTATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCAATC6040.20298631858769917No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5770.19391242686275234No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5750.1932402867349785No Hit
GTTGTCAATATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG5610.18853530584056166No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG5390.18114176443504942No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5210.17509250328508488No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5190.17442036315731105No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCTTCCTGC4850.1629939809851558No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4850.1629939809851558No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4810.1616497007296081No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGTTGGAATGTTGTGG4680.15728078989907815No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA4660.15660864977130431No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4610.15492829945186973No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC4570.15358401919632203No Hit
GTAGTAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4480.15055938862133977No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4420.14854296823801827No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4360.14652654785469676No Hit
CATGTATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4250.14282977715194065No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA4180.1404772867047322No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4120.1384608663214107No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4110.13812479625752377No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4100.13778872619363686No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4000.13442802555476766No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG3990.13409195549088074No Hit
ATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAAATAGA3990.13409195549088074No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3980.13375588542699382No Hit
CTATTAATAATGTCTTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG3920.13173946504367232No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3910.1314033949797854No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA3880.13039518478812465No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3810.1280426943409162No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG3800.12770662427702928No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACCC3760.12636234402148158No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3740.12569020389370775No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3730.12535413382982083No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3710.12468199370204701No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG3680.12367378351038624No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT3660.12300164338261241No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3630.12199343319095166No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC3630.12199343319095166No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCAACAAGCTCTGCTAG3570.11997701280763012No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3530.11863273255208245No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC3470.11661631216876095No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3460.11628024210487403No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC3450.1159441720409871No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3430.11527203191321327No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTCCT3390.1139277516576656No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3380.11359168159377868No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG3370.11325561152989176No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTG3370.11325561152989176No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3360.11291954146600484No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3340.11224740133823098No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3330.11191133127434406No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3330.11191133127434406No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC3320.11157526121045715No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3320.11157526121045715No Hit
GTCTTTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA3300.11090312108268331No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3300.11090312108268331No Hit
GGAACATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAA3270.10989491089102256No Hit
GGATTAAAGACAGGTACAGTAGTAGAATAAAGCCCAGTAAAGTTTCCCAC3260.10955884082713564No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3260.10955884082713564No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC3240.10888670069936181No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT3200.10754242044381412No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3200.10754242044381412No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3180.10687028031604029No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA3170.10653421025215337No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT3160.10619814018826645No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG3150.10586207012437954No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3100.10418171980494495No Hit
GGCCTATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG3070.10317350961328417No Hit
GTATGGATTGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3060.10283743954939725No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3060.10283743954939725No Hit
CCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATGT3060.10283743954939725No Hit
AAATAGACCTGTTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG3040.10216529942162342No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3020.10149315929384958No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3020.10149315929384958No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAAC3020.10149315929384958No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGGA3000.10082101916607575No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG3000.10082101916607575No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3000.10082101916607575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)