FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536487
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC25800.4809063406941827No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA23420.4365436627541767No Hit
GTGCTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAATTTGG19350.360679755520637No Hit
GCCTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC18010.33570244945357486No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG17360.32358659203298495No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT16980.31650347538710166No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16940.3157578841612192No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT16600.3094203587412183No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14920.27810552725415527No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT14090.26263450931709437No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCACGCCATGCAGTGGAA13230.24660429796062158No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC13040.24306273963767994No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA12880.24008037473415011No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG12810.23877559008885582No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12660.23597962299179662No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA12490.2328108602817962No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11780.21957661602238265No Hit
ATATGGAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA11600.21622145550591162No Hit
ATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG11490.21417107963473486No Hit
GAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA11320.21100231692473442No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11190.20857914544061645No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11060.20615597395649848No Hit
CCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATG10860.20242801782708622No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10830.2018688244076744No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10600.19758167485885025No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10470.19515850337473228No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA10430.19441291214884981No Hit
GCCTAGTCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC10420.19422651434237923No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGA10320.19236253627767308No Hit
CCTCTAAGGGACACTCATCCTCACGCCATGCAGTGGAACTCCACCACTTT10310.19217613847120246No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9960.185652215244731No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9890.1843474305994367No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC9750.18173786130884811No Hit
GTATTATCCAGAGTATGTAGTTGATCATTACTTCCAGACGCGACATTATT9720.18117866788943626No Hit
GTATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACACACTCTTT9450.1761459271147297No Hit
TCCTCACGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9320.17372275563061174No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAATTTGG9310.17353635782414112No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC9260.17260436879178806No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9140.17036759511414068No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9100.16962200388825824No Hit
CCCCAGCACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATG9100.16962200388825824No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8990.1675716280170815No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTGTCAACAATGTCCTCCTGC8770.163470876274728No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG8750.16309808066178677No Hit
CTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTT8710.1623524894359043No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC8700.1621660916294337No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8650.16123410259708065No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8610.16048851137119818No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8540.1591837267259039No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGCACGACAGAAT8390.15638775962884469No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA8340.1554557705964916No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8210.15303259911237363No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8200.15284620130590304No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA8160.15210061008002057No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG8140.15172781446707936No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG8120.15135501885413813No Hit
GAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTG7770.14483109562766666No Hit
CTCCTTCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7570.1411031394982544No Hit
TCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7520.1401711504659013No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCCCTCCCAAAAGTAAGA7480.13942555924001887No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7430.1384935702076658No Hit
GTACAGGGTCCCCAGTCTTCGATGAGATTGACGATATGGCAGAGACAGTA7370.1373751833688421No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7330.13662959214295967No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7220.13457921627178293No Hit
CCCCAACAGTCCCCCATGGGGAGGAGTGAACCCTGGCCCGAATGCTCCCA7040.13122405575531187No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6980.1301056689164882No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6970.12991927111001758No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT6950.12954647549707635No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6950.12954647549707635No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT6950.12954647549707635No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6920.1289872820776645No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG6910.1288008842711939No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGAACAGCAACATGAGGGA6790.12656411059354652No Hit
CTTTTGGAGTGTGGATTCGCACTCCTGCTCCATATAGACCACCAAATGCC6780.1263777127870759No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6750.12581851936766408No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6700.124886530335311No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT6650.12395454130295794No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6560.12227696104472244No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6550.1220905632382518No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6480.12078577859295753No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6480.12078577859295753No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6480.12078577859295753No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6410.11948099394766322No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6370.11873540272178076No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6340.11817620930236893No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6340.11817620930236893No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA6330.11798981149589831No Hit
CTCCTGCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6300.11743061807648648No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6200.11556664001178034No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6110.11388905975354482No Hit
GCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC6030.11239787730177991No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC6000.11183868388236808No Hit
CCTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCT6000.11183868388236808No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGGT5960.1110930926564856No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5960.1110930926564856No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5910.11016110362413255No Hit
ATCCAGAGTATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACAC5910.11016110362413255No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5900.10997470581766194No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5870.1094155123982501No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5860.10922911459177947No Hit
GGTCTATATGGAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA5850.10904271678530889No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5820.10848352336589703No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5750.10717873872060273No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT5710.10643314749472028No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT5710.10643314749472028No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5710.10643314749472028No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5690.10606035188177906No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG5610.10456916943001415No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5610.10456916943001415No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5590.1041963738170729No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5590.1041963738170729No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5580.10400997601060231No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5580.10400997601060231No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5510.10270519136530802No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG5510.10270519136530802No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5510.10270519136530802No Hit
GGGTAAAGGTTCAGATATTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA5470.10195960013942557No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG5470.10195960013942557No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5440.10140040672001371No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA5430.10121400891354311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)