FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536487
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC24250.4520146806912376No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA22020.4104479698482908No Hit
GTGCTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAATTTGG19670.36664448532769667No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG18830.35098706958416515No Hit
GCCTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC18280.34073519022828136No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT17510.32638255913004416No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15810.2946949320300399No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15680.29227176054592185No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14680.27363197989886057No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13250.24697709357356284No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA12770.23802999886297338No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCACGCCATGCAGTGGAA12730.23728440763709094No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12610.23504763395944356No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12490.2328108602817962No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA12230.22796451731356024No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC12160.22665973266826597No Hit
ATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG11350.21156151034414628No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11240.2095111344729695No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG11180.2083927476341458No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGA11140.20764715640826337No Hit
CCTCTAAGGGACACTCATCCTCACGCCATGCAGTGGAACTCCACCACTTT11030.20559678053708666No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10890.20298721124649804No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC10630.19814086827826208No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10620.1979544704717915No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA10270.19143054724532002No Hit
CCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATG10250.19105775163237879No Hit
ATATGGAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10220.19049855821296693No Hit
GCCTAGTCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC10190.1899393647935551No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10170.18956656918061388No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTGTCAACAATGTCCTCCTGC9570.17838270079237709No Hit
GAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA9570.17838270079237709No Hit
GTATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACACACTCTTT9550.17800990517943585No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG9440.17595952930825912No Hit
GTATTATCCAGAGTATGTAGTTGATCATTACTTCCAGACGCGACATTATT9290.17316356221119988No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9190.17129958414649377No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC9080.169249208275317No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAATTTGG9000.1677580258235521No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8900.16589404775884597No Hit
TCCTCACGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8890.16570764995237536No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC8880.16552125214590474No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC8800.16403006969413983No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG8730.16272528504884556No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8720.16253888724237492No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8640.16104770479061004No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8540.1591837267259039No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8500.15843813550002142No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC8460.15769254427413898No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG8400.1565741574353153No Hit
CCCCAGCACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATG8260.15396458814472672No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA8190.15265980349943242No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGCACGACAGAAT8110.1511686210476675No Hit
GAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTG8060.15023663201531445No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7990.14893184737002016No Hit
CTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTT7940.1479998583376671No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7930.14781346053119648No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCCCTCCCAAAAGTAAGA7740.1442719022082548No Hit
TCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7490.1396119570464895No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7420.13830717240119517No Hit
CTCCTGCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7310.13625679653001843No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGGT7290.1358840009170772No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7270.135511205304136No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7240.13495201188472414No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7150.1332744316264886No Hit
CCCCAACAGTCCCCCATGGGGAGGAGTGAACCCTGGCCCGAATGCTCCCA7140.13308803382001802No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7070.1317832491747237No Hit
GTACAGGGTCCCCAGTCTTCGATGAGATTGACGATATGGCAGAGACAGTA7030.13103765794884126No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7020.13085126014237064No Hit
CTCCTTCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6990.13029206672295882No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6940.12936007769060573No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6810.12693690620648776No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6800.12675050840001714No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6680.12451373472236978No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG6640.12376814349648733No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT6590.12283615446413428No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6530.12171776762531059No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT6520.12153136981883997No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6430.11985378956060445No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6430.11985378956060445No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6390.119108198334722No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6360.11854900491531015No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT6350.11836260710883954No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6350.11836260710883954No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT6340.11817620930236893No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG6340.11817620930236893No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6290.11724422027001587No Hit
CTTTTGGAGTGTGGATTCGCACTCCTGCTCCATATAGACCACCAAATGCC6280.11705782246354524No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6180.11519384439883912No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGAACAGCAACATGAGGGA6170.11500744659236851No Hit
GGTCTATATGGAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA6160.11482104878589788No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6140.11444825317295666No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC6010.11202508168883869No Hit
ATCCAGAGTATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACAC6010.11202508168883869No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG6000.11183868388236808No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5980.11146588826942685No Hit
CCTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCT5950.110906694850015No Hit
GCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC5940.11072029704354439No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG5890.10978830801119133No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5870.1094155123982501No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5870.1094155123982501No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5860.10922911459177947No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5850.10904271678530889No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5830.10866992117236764No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5790.1079243299464852No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5780.10773793214001456No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5740.10699234091413212No Hit
GGTTCAGATATTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5720.1066195453011909No Hit
GGGTAAAGGTTCAGATATTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA5690.10606035188177906No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTGACTT5650.1053147606558966No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5600.10438277162354354No Hit
CCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGG5600.10438277162354354No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5560.1036371803976611No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5520.10289158917177862No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5500.1025187935588374No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5460.10177320233295493No Hit
GTTTCCCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT5450.10158680452648433No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5410.10084121330060188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)