FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199040
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTTCCACTGCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGT13680.6872990353697749No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12870.6466036977491961No Hit
GGAATTCCACCACTTTCCACCAAACTCTTCAAGATCCCAGAGTCAGGGCC11570.581290192926045No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8310.41750401929260444No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7780.3908762057877813No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7660.38484726688102894No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6900.3466639871382637No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAAAGTCCAA6440.32355305466237944No Hit
ATATAGAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5870.2949155948553055No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC5550.278838424437299No Hit
GAATCATAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGTACCAAC4610.23161173633440513No Hit
GTTGCGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4570.22960209003215437No Hit
CTTCTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTTGCGT4070.2044815112540193No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3990.20046221864951766No Hit
CTCTTTGGAAGGCGGGGATCTTATATAGAAGAGAGTCCACACGTAGCGCC3950.1984525723472669No Hit
ACTCGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3950.1984525723472669No Hit
GTACCATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3880.19493569131832797No Hit
GTCCACTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC3840.19292604501607716No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG3750.18840434083601287No Hit
CCTCTAATTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTG3740.18790192926045016No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG3680.18488745980707397No Hit
GTCCAGAAGTACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3620.18187299035369775No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3580.17986334405144694No Hit
TCCAAAGAGTGTGTAAATAATGTTGCGTCTGGAAGTAATGATTAACTACA3570.17936093247588425No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCAACATTATT3510.17634646302250803No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3490.17534163987138263No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT3460.17383440514469453No Hit
GGAAGGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT3430.17232717041800644No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3280.16479099678456594No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3260.1637861736334405No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT3250.1632837620578778No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3190.1602692926045016No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3150.1582596463022508No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3130.1572548231511254No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT3110.15625No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3090.1552451768488746No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG3020.1517282958199357No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG2970.1492162379421222No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTAGGAAGTAATTTGG2950.14821141479099678No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2910.14620176848874597No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGT2900.14569935691318328No Hit
TACCTTGATAGTCCAGAAGTACCAACAAGAAGATGAGGCATAGCAGCAGG2890.14519694533762056No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG2820.14168006430868166No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG2780.13967041800643087No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA2760.13866559485530547No Hit
ATAGAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT2740.13766077170418006No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2740.13766077170418006No Hit
CTGTAAGGGGCCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC2710.13615353697749194No Hit
GTATGTAGTTAATCATTACTTCCAGACGCAACATTATTTACACACTCTTT2700.13565112540192928No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA2700.13565112540192928No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT2650.13313906752411575No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC2650.13313906752411575No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGTACAATGTGTT2640.13263665594855306No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCAACATTATTTACAC2620.13163183279742766No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGTTAAGGGAGTAGGCTGCCT2610.13112942122186494No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2560.12861736334405144No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG2560.12861736334405144No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG2550.12811495176848875No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGGCAATCAGGCA2550.12811495176848875No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA2530.12711012861736334No Hit
ATATGCAGGTGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA2520.12660771704180065No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG2480.12459807073954983No Hit
GAATTCCACCACTTTCCACCAAACTCTTCAAGATCCCAGAGTCAGGGCCC2470.12409565916398713No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTACGT2460.12359324758842445No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG2450.12309083601286175No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2440.12258842443729903No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT2440.12258842443729903No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA2430.12208601286173633No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA2430.12208601286173633No Hit
CTAATGGACCTGCCTCGTTGTCTAACAACAGTAGTTTCCGGAAGTGTTGA2420.12158360128617363No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG2410.12108118971061092No Hit
CTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC2410.12108118971061092No Hit
TCCTCAGGCCATGCAGTGGAAGTCCCCTGCTGGTGGCTCCAGTTCAGGAA2380.11957395498392283No Hit
CTTTTGGAGTGTGGATTCGCACTCCACCTGCATATAGACCACCAAATGCC2380.11957395498392283No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT2380.11957395498392283No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTTGCGTCTGGA2360.11856913183279742No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA2360.11856913183279742No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA2340.11756430868167203No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGT2330.11706189710610933No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG2300.11555466237942122No Hit
CTTTGGGACATGGACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGGA2290.11505225080385852No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA2290.11505225080385852No Hit
ATCTTATATAGAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACC2210.11103295819935692No Hit
ATGCAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG2200.11053054662379422No Hit
CCCCAACACAGAACAGCTTGCCTGATTGCCGTATGGTGAGCTGAACAATG2180.1095257234726688No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA2180.1095257234726688No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG2160.10852090032154342No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC2150.10801848874598072No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAAAGTAAGA2150.10801848874598072No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2140.107516077170418No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT2130.1070136655948553No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA2100.10550643086816722No Hit
CCATACGGCAATCAGGCAAGCTGTTCTGTGTTGGGGTGAGTTAATGAATC2090.1050040192926045No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA2060.10349678456591639No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCA2060.10349678456591639No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2040.102491961414791No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGGGGATTGGGGACAGAAAGAT2030.1019895498392283No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC2020.1014871382636656No Hit
CTCTAATTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC2000.10048231511254019No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC2000.10048231511254019No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG2000.10048231511254019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)