FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199040
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTTCCACTGCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGT11400.5727491961414791No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11220.5637057877813505No Hit
GGAATTCCACCACTTTCCACCAAACTCTTCAAGATCCCAGAGTCAGGGCC9140.45920418006430874No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7940.3989147909967846No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7630.38334003215434087No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7360.36977491961414793No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6400.3215434083601286No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAAAGTCCAA5810.29190112540192925No Hit
ATATAGAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4900.2461816720257235No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4720.23713826366559485No Hit
GTACCATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3980.19995980707395497No Hit
GTTGCGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG3960.1989549839228296No Hit
CTTCTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTTGCGT3950.1984525723472669No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3890.19543810289389066No Hit
ACTCGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3870.19443327974276528No Hit
GAATCATAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGTACCAAC3830.19242363344051447No Hit
CTCTTTGGAAGGCGGGGATCTTATATAGAAGAGAGTCCACACGTAGCGCC3640.18287781350482316No Hit
GTCCACTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC3610.18137057877813503No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3520.17684887459807075No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCAACATTATT3430.17232717041800644No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3420.17182475884244372No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT3370.16931270096463022No Hit
CCTCTAATTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTG3330.16730305466237944No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG3280.16479099678456594No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3260.1637861736334405No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3130.1572548231511254No Hit
TCCAAAGAGTGTGTAAATAATGTTGCGTCTGGAAGTAATGATTAACTACA3130.1572548231511254No Hit
GTCCAGAAGTACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3080.1547427652733119No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3050.1532355305466238No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG3000.15072347266881028No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG2980.1497186495176849No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGTTAAGGGAGTAGGCTGCCT2830.14218247588424437No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA2780.13967041800643087No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT2780.13967041800643087No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA2750.13816318327974278No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC2690.13514871382636656No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA2690.13514871382636656No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG2610.13112942122186494No Hit
TACCTTGATAGTCCAGAAGTACCAACAAGAAGATGAGGCATAGCAGCAGG2590.13012459807073953No Hit
CTGTAAGGGGCCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC2570.12911977491961416No Hit
GGAAGGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT2560.12861736334405144No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT2530.12711012861736334No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2520.12660771704180065No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG2520.12660771704180065No Hit
ATATGCAGGTGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA2500.12560289389067525No Hit
GTATGTAGTTAATCATTACTTCCAGACGCAACATTATTTACACACTCTTT2460.12359324758842445No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG2460.12359324758842445No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC2450.12309083601286175No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG2430.12208601286173633No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT2420.12158360128617363No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTAGGAAGTAATTTGG2420.12158360128617363No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA2380.11957395498392283No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAAAGTAAGA2360.11856913183279742No Hit
CTAATGGACCTGCCTCGTTGTCTAACAACAGTAGTTTCCGGAAGTGTTGA2360.11856913183279742No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG2360.11856913183279742No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2340.11756430868167203No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA2340.11756430868167203No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGGCAATCAGGCA2300.11555466237942122No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGT2290.11505225080385852No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCAACATTATTTACAC2290.11505225080385852No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGT2280.11454983922829583No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA2280.11454983922829583No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA2260.11354501607717042No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCA2240.11254019292604502No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA2240.11254019292604502No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC2230.11203778135048231No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA2230.11203778135048231No Hit
TCCTCAGGCCATGCAGTGGAAGTCCCCTGCTGGTGGCTCCAGTTCAGGAA2210.11103295819935692No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG2200.11053054662379422No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT2200.11053054662379422No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2200.11053054662379422No Hit
ATAGAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT2200.11053054662379422No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTTGCGTCTGGA2190.11002813504823152No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG2190.11002813504823152No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGGGGATTGGGGACAGAAAGAT2150.10801848874598072No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGTACAATGTGTT2140.107516077170418No Hit
CCATACGGCAATCAGGCAAGCTGTTCTGTGTTGGGGTGAGTTAATGAATC2090.1050040192926045No Hit
GAATTCCACCACTTTCCACCAAACTCTTCAAGATCCCAGAGTCAGGGCCC2080.1045016077170418No Hit
CTTTTGGAGTGTGGATTCGCACTCCACCTGCATATAGACCACCAAATGCC2080.1045016077170418No Hit
CCCCAACACAGAACAGCTTGCCTGATTGCCGTATGGTGAGCTGAACAATG2070.1039991961414791No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG2070.1039991961414791No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC2050.10299437299035369No Hit
CTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC2040.102491961414791No Hit
CTCTTCTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTTGC2040.102491961414791No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG2000.10048231511254019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)