FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403220
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGGACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC32120.7965874708595803No Hit
GTACCACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC27950.6931699816477357No Hit
GCTCAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC21630.5364317246168345No Hit
GTGTTGAGCAGATTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG20290.5031992460691433No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGACAAACTCGTG19550.4848469817965379No Hit
GTTGTAAGTAGGCCCTGAGCCTGAGTGCTCCACCCCAACATTCCCCCATG18460.45781459253013246No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT17300.42904617826496705No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16910.4193740389861614No Hit
GTGTGGTACAGATTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG16240.40275779971231584No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15980.3963097068597788No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT15770.3911016318634988No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15750.39060562472099597No Hit
GTTCAGTACAGGGTCCCCAATCGTCGATAAGATTGACGATGTGGCAGAGA13050.32364466048311097No Hit
ATATAAAAGAGAGTCCACTCGTAGCGCCTCATTTTGCGGGTCACCATATT12290.30479638906800255No Hit
GTCCCGTGCTGGTTGTCGATGATCCTGGAATTAGAGGACAAACGGGCAAC11800.29264421407668273No Hit
GCATCGGGAGGCCTTAGAGTCTCCCGAACATTGTACACCTCACCATACGG11150.27652398194534No Hit
AAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTACGT10480.25990774267149447No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9590.23783542483011758No Hit
CCCTTAACTCCACCTCTAAGGGAAACTCATCCTCAGGCCGCGCAATGGAA9380.2326273498338376No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATCAACTACA9000.2232032141262834No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA8990.22295521055503198No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8920.221219185556272No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACTCGTAGCGCC8890.22047517484251772No Hit
TACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTCGCCCC8640.21427508556123206No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8520.211299042706215No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGGACCTCTATG8330.20658697485243788No Hit
GTACTGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT8220.20385893556867218No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC8060.19989087842864936No Hit
GCATAGGGAGGCCTTAGAGTCTCCCGAACATTGTACACCTCACCATACGG7930.19666683200238083No Hit
GTTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA7860.19493080700362084No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7830.19418679628986657No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT7760.1924507712911066No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC7550.18724269629482665No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7470.18525866772481522No Hit
GTATTATCCAGAGCATGTAGTTGATCATTACTTCCAGACGAGACATTATT7430.18426665343980955No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCGACAACC7390.18327463915480383No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAGTCAA7270.18029859629978673No Hit
ATCCAGAGCATGTAGTTGATCATTACTTCCAGACGAGACATTATTTACAC7250.17980258915728387No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7240.17955458558603243No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7050.17484251773225534No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7000.1736024998759982No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6820.16913843559347255No Hit
GGTAGAGGCAGGAGGAGGAGCTGCTGGCACAGTTGTAAGTAGGCCCTGAG6560.16269034274093547No Hit
GCCTCCACCAATCGGCAGTCAGGAAGGCAGCCTACTCCCTTAACTCCACC6340.15723426417340408No Hit
GTTTGGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6130.15202618917712415No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6090.15103417489211846No Hit
CTCCAAAAGACACCAAATAGTCAAGAACAGTTTGTCTCCCAAAAGTAATA6070.1505381677496156No Hit
ACATAGAGGTCCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTG6050.15004216060711273No Hit
TTGTACACCTCACCATACGGCAATCAGGCAAGCTGTTCTATGTTGGGGTG6040.14979415703586133No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6040.14979415703586133No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6020.14929814989335846No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5970.14805813203710133No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACGT5960.14781012846584993No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAGTCAAGAA5940.14731412132334704No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGTAT5940.14731412132334704No Hit
GTGGTACAGATTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCC5920.1468181141808442No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5900.14632210703834136No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5810.1440900748970785No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCTACCAATCGGCAGT5810.1440900748970785No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5740.14235404989831854No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCTTGGCTCAGTTTACTAGTG5740.14235404989831854No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGA5680.14086602847080998No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5650.1401220177570557No Hit
TCTGCTCAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTT5580.13838599275829572No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5570.1381379891870443No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5540.13739397847329002No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5420.1344179356182729No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5340.1324339070482615No Hit
ACTACATGCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5260.13044987847825007No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5230.12970586776449583No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5220.12945786419324437No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5200.12896185705074154No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5140.12747383562323297No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5130.12722583205198154No Hit
ACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCT5110.1267298249094787No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5090.12623381776697584No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG5050.12524180348197014No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5000.124001785625713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4990.1237537820544616No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTGTGCCAACGAATTGTGGGTCT4990.1237537820544616No Hit
TCCTCAGGCCGCGCAATGGAATTCCACCACTTTCCACCAAACTCTTCAAG4960.12300977134070731No Hit
GTCGGAACGACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG4920.12201775705570159No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4910.12176975348445018No Hit
TCCTAGTACAAAGACCTTTAACCTCGTCTCCTCCCCCAACTCCTCCCACT4900.12152174991319875No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4900.12152174991319875No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4840.12003372848569019No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4810.11928971777193592No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4750.11780169634442736No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4700.11656167848817023No Hit
CCCGCCAGTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG4700.11656167848817023No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4690.1163136749169188No Hit
ATAAAAGAGAGTCCACTCGTAGCGCCTCATTTTGCGGGTCACCATATTCT4680.11606567134566739No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4660.11556966420316452No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4650.11532166063191311No Hit
GTTCGGTACAGGGTCCCCAATCGTCGATAAGATTGACGATGTGGCAGAGA4620.11457764991815883No Hit
ATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4560.11308962849065027No Hit
GCCTGAGGATGAGTTTCCCTTAGAGGTGGAGTTAAGGGAGTAGGCTGCCT4540.11259362134814742No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4510.11184961063439314No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT4500.1116016070631417No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4490.11135360349189029No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC4420.1096175784931303No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4400.10912157135062744No Hit
ATGCAAGACCTTGGGCAAGTTTCCGAGGGCGTTCACGGTGGTTTCCATGC4280.10614552849561035No Hit
CCTCTAAGGGAAACTCATCCTCAGGCCGCGCAATGGAATTCCACCACTTT4260.1056495213531075No Hit
CCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAGT4260.1056495213531075No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4240.10515351421060462No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG4230.10490551063935322No Hit
CCCCTATCTTATCAACACTTCCGGAAGTTACTGTTGTTAGACCAAGAGGC4210.10440950349685034No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC4120.10217747135558752No Hit
GCCTTAGAGTCTCCCGAACATTGTACACCTCACCATACGGCAATCAGGCA4120.10217747135558752No Hit
CTCTTATGCAAGACCTTGGGCAAGTTTCCGAGGGCGTTCACGGTGGTTTC4110.10192946778433609No Hit
GGGTAAAGGTTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA4100.10168146421308467No Hit
GCCTATAGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC4090.10143346064183324No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4050.10044144635682754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)