FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403220
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGGACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC30530.7571549030306036No Hit
GTACCACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC28080.6963940280740042No Hit
GCTCAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC21950.5443678388968801No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGACAAACTCGTG21760.539655771043103No Hit
GTGTTGAGCAGATTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG19340.4796389068002579No Hit
GTTGTAAGTAGGCCCTGAGCCTGAGTGCTCCACCCCAACATTCCCCCATG17950.4451664103963097No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT17410.43177421754873274No Hit
GTGTGGTACAGATTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG16300.4042458211398244No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15860.39333366400476166No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT15440.3829175140122018No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15380.3814294925846932No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15030.37274936759089333No Hit
ATATAAAAGAGAGTCCACTCGTAGCGCCTCATTTTGCGGGTCACCATATT12240.30355637121174545No Hit
GTTCAGTACAGGGTCCCCAATCGTCGATAAGATTGACGATGTGGCAGAGA11840.2936362283616884No Hit
GCATCGGGAGGCCTTAGAGTCTCCCGAACATTGTACACCTCACCATACGG11520.28570011408164275No Hit
GTCCCGTGCTGGTTGTCGATGATCCTGGAATTAGAGGACAAACGGGCAAC11410.2829720747978771No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA10600.2628837855265116No Hit
CCCTTAACTCCACCTCTAAGGGAAACTCATCCTCAGGCCGCGCAATGGAA9910.2457715391101632No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACTCGTAGCGCC9710.24081146768513464No Hit
AAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTACGT8820.21873914984375775No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGGACCTCTATG8720.2162591141312435No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8680.21526709984623782No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC8340.2068349784236893No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT8230.20410693913992364No Hit
GTACTGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT8160.20237091414116362No Hit
GTTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA8070.20013888199990082No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA8060.19989087842864936No Hit
GCATAGGGAGGCCTTAGAGTCTCCCGAACATTGTACACCTCACCATACGG8000.1984028570011408No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATCAACTACA7930.19666683200238083No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7880.19542681414612373No Hit
GTATTATCCAGAGCATGTAGTTGATCATTACTTCCAGACGAGACATTATT7820.19393879271861514No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7810.1936907891473637No Hit
TACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTCGCCCC7650.1897227320073409No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7630.18922672486483805No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAGTCAA7420.1840186498685581No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7100.17608253558851247No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6980.17310649273349538No Hit
GTTTGGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6790.16839442487971826No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6660.16517037845344973No Hit
GCCTCCACCAATCGGCAGTCAGGAAGGCAGCCTACTCCCTTAACTCCACC6530.1619463320271812No Hit
ATCCAGAGCATGTAGTTGATCATTACTTCCAGACGAGACATTATTTACAC6480.16070631417092407No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6440.15971429988591834No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCGACAACC6410.15897028917216408No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6240.15475422846088985No Hit
GTGGTACAGATTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCC6010.14905014632210703No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6000.1488021427508556No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5940.14731412132334704No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5930.14706611775209563No Hit
TTGTACACCTCACCATACGGCAATCAGGCAAGCTGTTCTATGTTGGGGTG5860.14533009275333567No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5710.14161003918456425No Hit
TCTGCTCAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTT5700.14136203561331284No Hit
CTCCAAAAGACACCAAATAGTCAAGAACAGTTTGTCTCCCAAAAGTAATA5670.14061802489955855No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5660.14037002132830712No Hit
TCCTCAGGCCGCGCAATGGAATTCCACCACTTTCCACCAAACTCTTCAAG5650.1401220177570557No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5600.13888199990079855No Hit
GGTAGAGGCAGGAGGAGGAGCTGCTGGCACAGTTGTAAGTAGGCCCTGAG5590.13863399632954715No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5560.13788998561579285No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGA5550.13764198204454142No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5520.13689797133078718No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5480.13590595704578146No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAGTCAAGAA5470.13565795347453002No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACGT5460.13540994990327862No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCTACCAATCGGCAGT5440.13491394276077576No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5340.1324339070482615No Hit
ACATAGAGGTCCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTG5310.13168989633450723No Hit
GTCGGAACGACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG5220.12945786419324437No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5220.12945786419324437No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5220.12945786419324437No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCTTGGCTCAGTTTACTAGTG5090.12623381776697584No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5060.12548980705322157No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5050.12524180348197014No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5030.12474579633946728No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4990.1237537820544616No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4920.12201775705570159No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4900.12152174991319875No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4830.11978572491443877No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGTAT4770.11829770348693021No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4760.11804969991567878No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4730.11730568920192451No Hit
GCCTTAGAGTCTCCCGAACATTGTACACCTCACCATACGGCAATCAGGCA4710.11680968205942165No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4700.11656167848817023No Hit
ATAAAAGAGAGTCCACTCGTAGCGCCTCATTTTGCGGGTCACCATATTCT4650.11532166063191311No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4630.11482565348941023No Hit
ACTACATGCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4620.11457764991815883No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4580.1135856356331531No Hit
CCCGCCAGTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG4560.11308962849065027No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4540.11259362134814742No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4520.11209761420564457No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4510.11184961063439314No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4470.11085759634938744No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT4460.110609592778136No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG4420.1096175784931303No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTGTGCCAACGAATTGTGGGTCT4370.10837756063687316No Hit
CCCTTAACTTCATGGGATATGTAATTGGGGGTTGGGGCACATTGCCACAG4350.10788155349437031No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4310.10688953920936463No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA4310.10688953920936463No Hit
ATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4310.10688953920936463No Hit
GTTCGGTACAGGGTCCCCAATCGTCGATAAGATTGACGATGTGGCAGAGA4250.10540151778185607No Hit
TCCTAGTACAAAGACCTTTAACCTCGTCTCCTCCCCCAACTCCTCCCACT4250.10540151778185607No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC4240.10515351421060462No Hit
ACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCT4240.10515351421060462No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4230.10490551063935322No Hit
GCTATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTCTCCTCTGCCGAT4210.10440950349685034No Hit
GCCTGAGGATGAGTTTCCCTTAGAGGTGGAGTTAAGGGAGTAGGCTGCCT4190.1039134963543475No Hit
CCTCTAAGGGAAACTCATCCTCAGGCCGCGCAATGGAATTCCACCACTTT4180.10366549278309609No Hit
GAATTTGGAGCTTCTACTGAGTTACTCTCTTTTTTGCCTTCTGACTTCTT4170.10341748921184465No Hit
GTATAAAGAGTTTGGAGCTTCTACTGAGTTACTCTCTTTTTTGCCTTCTG4140.10267347849809037No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4130.10242547492683896No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA4070.10093745349933039No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG4070.10093745349933039No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4060.10068944992807896No Hit
GGGTAAAGGTTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA4050.10044144635682754No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC4050.10044144635682754No Hit
CTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACTCGTAGCGCCTC4040.10019344278557611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)