FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467695
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21490.45948748650295596No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21100.45114871871625745No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21000.44901057312992443No Hit
GTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAA17110.3658367098215718No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA16340.34937298880680784No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13540.2895049123894846No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG13300.28437336298228544No Hit
GTTAATATTCCTTGGACACACAAGGTGGGAAACTTTACGGGGCTTTATTC12580.2689787147606881No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12150.25978468873945626No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGTCGTTCACGGTGGTTTCCATGC11460.24503148419375875No Hit
CTTTTATATAAGATACCCGCTTTCCAAAGAGTGTGTAAATAATGTCTCGT11160.23861704743475984No Hit
AAAAATCAGACAATTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA10760.23006446508942796No Hit
ATATAAAAGAGAGTCCACACGTAGTGCCTCATTTTACGGGTCACCATATT10660.227926319503095No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC10640.2274986903858284No Hit
ATATGGAGGAGGAGTGCGTATCCACACTCCAAAAGACACCAAATATTCAA9600.20526197628796544No Hit
CTCAGACTACTGTCTCGGCCATATCGTCAATCTTATCGAAGACTGGGGAC9450.20205475790846597No Hit
CTAAAGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTC9410.20119949967393277No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9180.19628176482536694No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8960.19157784453543442No Hit
GTTAGTATTCCTTGGACACACAAGGTGGGAAACTTTACGGGGCTTTATTC8930.19093640085953453No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8170.17468649440340392No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8150.17425886528613732No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8110.17340360705160413No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8060.17233453425843764No Hit
GTATAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC8010.17126546146527116No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7890.1686996867616716No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7620.16292669367857257No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7560.16164380632677278No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7360.15736751515410685No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAACAGCCCCCCATG7230.15458792589187398No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT7130.15244978030554102No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT7020.15009782016057474No Hit
CTCTTTGGAAAGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGTGCC6950.14860111825014166No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6840.1462491581051754No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6800.1453938998706422No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6790.1451800853120089No Hit
CCTTCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCATTCAGGATTAAAG6720.14368338340157583No Hit
GTTGGGATGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGCAG6630.14175905237387615No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6500.13897946311164328No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6390.136627502966677No Hit
CCTCCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6300.13470317193897732No Hit
GGGATGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGCAGCTC6210.13277884091127765No Hit
GGTATGAATCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGG6170.13192358267674448No Hit
CCCATATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6160.13170976811811116No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6150.13149595355947788No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6130.13106832444221128No Hit
ATATGTTCCTGAGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6110.13064069532494468No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5950.12721966238681193No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5870.12550914591774553No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5870.12550914591774553No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5860.12529533135911225No Hit
TTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTAC5850.12508151680047896No Hit
CATAGCTGACTACTGCTTCCCTGGATGTTGGATCGTCTAAATTACCTCCC5830.12465388768321235No Hit
CAGACTACTGTCTCGGCCATATCGTCAATCTTATCGAAGACTGGGGACCC5780.12358481489004587No Hit
ACAACGAGGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAA5710.12208811297961279No Hit
CATTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5630.12037759651054641No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5630.12037759651054641No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5620.12016378195191312No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5560.11888089460011333No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAGT5520.11802563636558014No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5460.11674274901378036No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCATTTATACC5400.11545986166198058No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5370.11481841798608068No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5350.11439078886881408No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5220.11161119960658122No Hit
GTCACCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAATTATTGTG5200.11118357048931463No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5190.11096975593068133No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5160.11032831225478144No Hit
CTTTTGGAGTGTGGATACGCACTCCTCCTCCATATAGACCACCAAATGCC5150.11011449769614814No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5140.10990068313751485No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACTT5130.10968686857888155No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT5110.10925923946161495No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5050.10797635210981514No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAA5010.10712109387528196No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4980.10647965019938206No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACT4970.10626583564074876No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4930.10541057740621558No Hit
CTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTC4920.10519676284758228No Hit
GTCCAACTGATGGTCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4910.10498294828894898No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG4900.10476913373031568No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4890.10455531917168238No Hit
GGCAAAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAA4890.10455531917168238No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCCGAGACAGTA4840.1034862463785159No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATATGGGAGTAGGCTGCCT4820.1030586172612493No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA4820.1030586172612493No Hit
GGCTTTAGAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAA4790.10241717358534942No Hit
AGTCTGTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTT4750.10156191535081623No Hit
CTGACCTTTAGGCCCATATTAACGTTGACATAGCTGACTACTGCTTCCCT4720.10092047167491634No Hit
CAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGA4710.10070665711628304No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGTCGTTC4690.10027902799901645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)