FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467695
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21440.45841841370978953No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17480.3737478484910038No Hit
GTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAA15810.33804081719924306No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA15060.3220047253017458No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14300.3057548188456152No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG13020.2783865553405531No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12470.2666267546157218No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11890.25422551021499057No Hit
GTTAATATTCCTTGGACACACAAGGTGGGAAACTTTACGGGGCTTTATTC10950.2341269417034606No Hit
CTTTTATATAAGATACCCGCTTTCCAAAGAGTGTGTAAATAATGTCTCGT10750.22985065053079465No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC9980.21338692951603072No Hit
CTAAAGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTC9890.21146259848833104No Hit
ATATGGAGGAGGAGTGCGTATCCACACTCCAAAAGACACCAAATATTCAA9720.207827750991565No Hit
ATATAAAAGAGAGTCCACACGTAGTGCCTCATTTTACGGGTCACCATATT9670.20675867819839852No Hit
AAAAATCAGACAATTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA9460.20226857246709926No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGTCGTTCACGGTGGTTTCCATGC9420.2014133142325661No Hit
CTCAGACTACTGTCTCGGCCATATCGTCAATCTTATCGAAGACTGGGGAC8900.19029495718363465No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8760.1873015533627685No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8550.18281144763146923No Hit
GTTAGTATTCCTTGGACACACAAGGTGGGAAACTTTACGGGGCTTTATTC8170.17468649440340392No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8130.17383123616887072No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7810.1669891702926052No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7750.16570628294080544No Hit
GTATAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC7660.16378195191310577No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7610.16271287911993926No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7560.16164380632677278No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7400.15822277338864002No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7210.15416029677460738No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT6910.14774586001560847No Hit
CCTTCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCATTCAGGATTAAAG6740.1441110125188424No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT6460.13812420487711008No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6410.1370551320839436No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6390.136627502966677No Hit
CTCTTTGGAAAGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGTGCC6290.13448935738034404No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6280.13427554282171072No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAACAGCCCCCCATG6150.13149595355947788No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6080.1299992516490448No Hit
GTTGGGATGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGCAG6080.1299992516490448No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6000.1282887351799784No Hit
ATATGTTCCTGAGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5940.12700584782817861No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5930.12679203326954533No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5850.12508151680047896No Hit
TTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTAC5790.12379862944867917No Hit
GGTATGAATCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGG5770.12337100033141257No Hit
CCTCCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5700.1218742984209795No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5650.12080522562781301No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATGA5550.11866708004148004No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5540.11845326548284674No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAGT5480.11717037813104696No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5470.11695656357241366No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5420.11588749077924716No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5410.11567367622061386No Hit
CCCATATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5410.11567367622061386No Hit
CATAGCTGACTACTGCTTCCCTGGATGTTGGATCGTCTAAATTACCTCCC5400.11545986166198058No Hit
CATTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5370.11481841798608068No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5330.1139631597515475No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5310.1135355306342809No Hit
GGGATGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGCAGCTC5310.1135355306342809No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5280.112894086958381No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5270.1126802723997477No Hit
CTTTTGGAGTGTGGATACGCACTCCTCCTCCATATAGACCACCAAATGCC5230.11182501416521452No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATATGGGAGTAGGCTGCCT5220.11161119960658122No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACTT5220.11161119960658122No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5210.11139738504794792No Hit
ACAACGAGGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAA5150.11011449769614814No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCATTTATACC5150.11011449769614814No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5130.10968686857888155No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5010.10712109387528196No Hit
GTGGATATCCTGCTCTAAAGCCTTTATATGCATGTATACAAGCAAAACAG4990.10669346475801536No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4950.10583820652348218No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4920.10519676284758228No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4910.10498294828894898No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT4890.10455531917168238No Hit
CTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTC4880.1043415046130491No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGACCATCAGTTG4840.1034862463785159No Hit
GCACAAACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACAGTT4830.1032724318198826No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4830.1032724318198826No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4820.1030586172612493No Hit
GTCCAACTGATGGTCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4800.10263098814398272No Hit
CAGACTACTGTCTCGGCCATATCGTCAATCTTATCGAAGACTGGGGACCC4790.10241717358534942No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT4730.10113428623354963No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4730.10113428623354963No Hit
TCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCA4710.10070665711628304No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4680.10006521344038315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)