FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418359
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17300.4135204453591294No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAAAGTCCAA16370.3912907335565866No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16340.3905736460790852No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13140.3140843151456046No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12090.2889862534330563No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAATTCCCGTG12080.28874722427388916No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTTCGGGTCACCATATT11870.2837276119313795No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG11660.2787079995888698No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT10530.2516977046029845No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9860.23568275093878702No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9690.23161925523294585No Hit
CTCCAAAAGAGACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA9520.2275557595271047No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC9380.22420935129876496No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA9170.21918973895625526No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATCAGAGGACAAACGGGCAAC8800.2103456600670716No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8450.20197963949622214No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC8430.20150158117788786No Hit
ATTCTGAGCAGGGCTCAATGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7950.1900281815378658No Hit
GAATACTAGCATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA7740.18500856919535616No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7590.18142313180784925No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7540.18022798601201362No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA7460.1783157527386766No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7450.17807672357950946No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7440.17783769442034233No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7010.16755944057615588No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6970.16660332393948737No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6940.165886236461986No Hit
ACCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6680.1596714783236407No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6590.15752021589113654No Hit
GTATACATCCTTCCCATGGCTGCTAGGTTGTGCTGCCAACTGGATCCTGC6560.15680312841363517No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCATCAACAACC6520.1558470117769667No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6460.1544128368219639No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6370.15226157438945978No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6320.15106642859362412No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6210.14843710784278574No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6190.1479590495244515No Hit
ATACTGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTAATGTCTCTA6150.147002932887783No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6050.1446126412961117No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGT6020.14389555381861033No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6020.14389555381861033No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5930.1417442913861062No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5880.14054914559027057No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5800.13863691231693354No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT5770.13791982483943216No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5770.13791982483943216No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5750.1374417665210979No Hit
TCACCATACTGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTAATGT5660.13529050408859375No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5650.13505147492942662No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5630.1345734166110924No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5530.13218312501942112No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5500.13146603754191974No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5410.1293147751094156No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCACGCATGCGCTGATGGCC5340.1276415709952457No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAA5300.1266854543585772No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAG5300.1266854543585772No Hit
ATTTTGTGTTGGGGTGAGTTAATGTCTCTAGCCACCTGGGTGGGAAGTAA5250.12549030856274157No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5250.12549030856274157No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5220.12477322108524018No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA5210.12453419192607305No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5170.12357807528940457No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5150.1231000169710703No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5130.12262195865273605No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5120.12238292949356892No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5080.1214268128569004No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4960.11855846294689489No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATC4870.11640720051439075No Hit
GTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4790.11449496724105374No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4750.11353885060438522No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4750.11353885060438522No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4690.11210467564938248No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4660.11138758817188109No Hit
CTATTGTGGTTTCACATTTCCTGTCTTTCTTTTGGGAGAGAAACTGTTCT4650.11114855901271396No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTTCGGTGGGCGTTC4640.11090952985354684No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGAGTGGCTTTAGGACATG4620.11043147153521257No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4510.10780215078437419No Hit
ACACCTCACCATACTGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTT4490.10732409246603992No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4470.10684603414770567No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGA4460.10660700498853855No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAAGAA4460.10660700498853855No Hit
GAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4460.10660700498853855No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTG4400.1051728300335358No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4400.1051728300335358No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTATGCC4390.10493380087436867No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA4330.1034996259193659No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAACGAAGGAGTTT4310.10302156760103165No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA4290.10254350928269738No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4250.1015873926460289No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTA4210.10063127600936038No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4200.10039224685019325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)