FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418359
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16290.38937850028324955No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15830.3783831589615617No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAAAGTCCAA14040.335596939470646No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAATTCCCGTG13140.3140843151456046No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12700.30356703214225106No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG11020.26341013340217373No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10870.2598246960146668No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTTCGGGTCACCATATT10850.25934663769633254No Hit
CTCCAAAAGAGACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA10000.2390291591671268No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9900.23663886757545552No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT9800.23424857598378424No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9340.22325323466209643No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC8530.20389187276955914No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA8440.201740610337055No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATCAGAGGACAAACGGGCAAC7900.18883303574203017No Hit
GAATACTAGCATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA7670.18333536508118625No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7540.18022798601201362No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7350.1756864319878382No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7300.17449128619200255No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7210.17234002375949842No Hit
GTATACATCCTTCCCATGGCTGCTAGGTTGTGCTGCCAACTGGATCCTGC7200.1721009946003313No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7190.17186196544116417No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA6950.16612526562115312No Hit
ATTCTGAGCAGGGCTCAATGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6940.165886236461986No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6840.16349594487031474No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6530.15608604093613382No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6400.15297866186696113No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6330.15130545775279128No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6090.14556875793278024No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6050.1446126412961117No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6030.14413458297777745No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5990.14317846634110895No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5960.14246137886360757No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5780.13815885399859928No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5740.13720273736193078No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5730.13696370820276366No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5670.13552953324776087No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5660.13529050408859375No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAG5590.1336172999744239No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5490.1312270083827526No Hit
ACCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5460.13050992090525124No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5460.13050992090525124No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5440.13003186258691699No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCATCAACAACC5430.12979283342774986No Hit
ATACTGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTAATGTCTCTA5430.12979283342774986No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5360.12811962931357995No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5350.12788060015441283No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5290.12644642519941007No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5260.1257293377219087No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAA5250.12549030856274157No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5180.12381710444857169No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5160.12333904613023744No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5130.12262195865273605No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5120.12238292949356892No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5080.1214268128569004No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA5020.11999263790189767No Hit
TCACCATACTGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTAATGT4990.11927555042439628No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4940.11808040462856063No Hit
ATTTTGTGTTGGGGTGAGTTAATGTCTCTAGCCACCTGGGTGGGAAGTAA4910.11736331715105924No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGT4910.11736331715105924No Hit
CTATTGTGGTTTCACATTTCCTGTCTTTCTTTTGGGAGAGAAACTGTTCT4880.1166462296735579No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4850.1159291421960565No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4790.11449496724105374No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCACGCATGCGCTGATGGCC4780.11425593808188661No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTG4680.11186564649021534No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA4660.11138758817188109No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGAGTGGCTTTAGGACATG4640.11090952985354684No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT4620.11043147153521257No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTATGCC4610.11019244237604545No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4550.10875826742104269No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4510.10780215078437419No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4470.10684603414770567No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA4410.10541185919270292No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTA4330.1034996259193659No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATC4330.1034996259193659No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4330.1034996259193659No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG4330.1034996259193659No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4280.10230448012353027No Hit
TCCTCAAGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4270.10206545096436315No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4240.10134836348686177No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4210.10063127600936038No Hit
GTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4210.10063127600936038No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGA4200.10039224685019325No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4200.10039224685019325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)