FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492911
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG21430.4347640851999651No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20370.4132591887785016No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT16590.3365719166340374No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT16370.33210863624467707No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC13840.28078091176703296No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA13500.27388311480165795No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11860.24061138826278983No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCT11460.2324963330094074No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11140.22600428880670143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11100.2251927832813632No Hit
CATACAACGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA11090.2249899069000286No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCTTTGACATACT10940.2219467611800102No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT10260.20815116724926No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10170.20632527981724896No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGTCCGTTCACGGTGGTCTCCATGC9630.19536995522518263No Hit
CTTGACTCCCTTTACACCGCTATTACCAATTTTCTTTTGTCTTTGGGTAT9540.19354406779317157No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACTCACG9400.19070379845448773No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9350.18968941654781493No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATA9090.1844146306331163No Hit
CCTTTACACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA9060.18380600148911264No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8990.1823858668197707No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATCAATAATGTCCTCCTGT8950.18157436129443247No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTAATGACTTCTTTCCTTCTATTCG8330.16899602565168967No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG8240.1671701382196786No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT7870.1596637121102998No Hit
GTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGC7670.1556061844836086No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7640.1549975553396049No Hit
CTATTCTTCTTCTGTACCTATTTTTAATTCTGAGTGGCAAACTCCCTCCT7330.1487083875182335No Hit
ATATTAACGTTGACATAGCTGACCACTGCTTCCCGGGATGTTGGATCTTC7040.14282497245953124No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6930.14059333226485107No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA6730.13653580463815984No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6600.13389841168081054No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6290.12760924385943911No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6270.12720349109677No Hit
CCTGCCGCTTACAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGA6270.12720349109677No Hit
CTGCAGAACTCCTGGCAGCTTGTTTTGCTCGCAGCCGGTCTGGGGCAAAA6270.12720349109677No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTCTATTC6160.12497185090208983No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA6160.12497185090208983No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6150.12476897452075528No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6010.12192870518207141No Hit
ATATGCTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5940.12050857051272947No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5930.12030569413139491No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5900.11969706498739124No Hit
CTCATAAGGTGGGAAACTTTACTGGACTCTATTCTTCTTCTGTACCTATT5620.11401652631002353No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5510.11178488611534333No Hit
ATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGC5490.11137913335267421No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5470.1109733805900051No Hit
GGGACTCAAGGTGTTGTACAGATTTGGCCCCCAATACCACATCATCCATA5470.1109733805900051No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGATAG5420.1099589986833323No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC5410.10975612230199772No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5370.10894461677665948No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT5340.10833598763265578No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5300.10752448210731755No Hit
GCACACCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAAC5170.10488708914996826No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5170.10488708914996826No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5150.10448133638729913No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTCTATTCTTCTTCTG5010.10164106704861527No Hit
CTCCAAAAGACACCAAAAACTCAAGTACAGTATCTCTTCCAAAAGTAAGA5010.10164106704861527No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4980.10103243790461158No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4970.10082956152327703No Hit
TCACCATACAGCACTCAGGCAAGAGATTCTATGTTGGGGTGAGTTGATGA4960.10062668514194245No Hit
CGTTAATATGGGCCTGAAACTTAGACAACTATTGTGGTTTCACATTTCCT4930.10001805599793878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)