FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492911
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG24440.49582987598166806No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20290.4116361777278251No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT16260.3298769960499969No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15280.3099951106792098No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC13450.2728687328949851No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA13360.271042845462974No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12030.2440602867454774No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11960.24264015207613546No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCTTTGACATACT10840.2199179973666646No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACTCACG10830.21971512098533003No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCT10660.21626622250264246No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10620.2154547169773042No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT10580.21464321145196597No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTAATGACTTCTTTCCTTCTATTCG9550.19374694417450616No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9470.19212393312382964No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9450.19171818036116053No Hit
CATACAACGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA9210.18684914720913107No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATA9050.1836031251077781No Hit
CTTGACTCCCTTTACACCGCTATTACCAATTTTCTTTTGTCTTTGGGTAT8830.17913984471841773No Hit
CTATTCTTCTTCTGTACCTATTTTTAATTCTGAGTGGCAAACTCCCTCCT8730.1771110809050721No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATCAATAATGTCCTCCTGT8730.1771110809050721No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8440.17122766584636984No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8400.1704161603210316No Hit
CCTTTACACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7860.15946083572896527No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG7850.1592579593476307No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGTCCGTTCACGGTGGTCTCCATGC7840.15905508296629614No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7720.1566205663902814No Hit
GTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGC7450.15114290409424827No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT7200.14607099456088424No Hit
ATATTAACGTTGACATAGCTGACCACTGCTTCCCGGGATGTTGGATCTTC7170.1454623654168805No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA7170.1454623654168805No Hit
CCTGCCGCTTACAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGA6610.13410128806214508No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6390.12963800767278474No Hit
CTGCAGAACTCCTGGCAGCTTGTTTTGCTCGCAGCCGGTCTGGGGCAAAA6370.12923225491011564No Hit
ATATGCTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6320.1282178730034428No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6280.12740636747810458No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6250.12679773833410088No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC6010.12192870518207141No Hit
GGGACTCAAGGTGTTGTACAGATTTGGCCCCCAATACCACATCATCCATA5890.11949418860605666No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA5890.11949418860605666No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5870.11908843584338756No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5860.11888555946205298No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5790.11746542479271105No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5780.1172625484113765No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5700.11563953736070001No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGATAG5680.11523378459803088No Hit
CTCCAAAAGACACCAAAAACTCAAGTACAGTATCTCTTCCAAAAGTAAGA5620.11401652631002353No Hit
ATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGC5470.1109733805900051No Hit
CTCATAAGGTGGGAAACTTTACTGGACTCTATTCTTCTTCTGTACCTATT5440.1103647514460014No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5430.11016187506466685No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5400.10955324592066316No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5350.10853886401399036No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTCTATTC5280.10711872934464843No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5230.10610434743797562No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTTCGAAAAC5230.10610434743797562No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5210.1056985946753065No Hit
GTATAAAGAATTTGGAGCTTCTTTGGAGTTACTCTCTTTTTTGCCTAATG5170.10488708914996826No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5170.10488708914996826No Hit
GCACACCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAAC5090.10326407809929175No Hit
GGTCTATTTACAGGAAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT5070.10285832533662265No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5030.10204681981128438No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4990.10123531428594613No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4970.10082956152327703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)