FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474501
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46100.9715469514289748No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG19990.42128467590163143No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAACCTTAC14850.3129603520329778No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG10610.2236033222269289No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10140.21369817977201314No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA9840.20737574841781153No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9750.20547901901155108No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9520.20063182163999657No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9490.19999957850457636No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC9370.19747060596289578No Hit
CTACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9250.19494163342121512No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAA8880.1871439680843665No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC8580.18082153673016496No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTGCAGG8480.17871405961209777No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8470.17850331190029106No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8250.1738668622405432No Hit
ATCTTAACCTCTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG8020.1690196648689887No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7860.16564770148008118No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAAC7810.16459396292104758No Hit
AGTCTGTACAACACCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7800.16438321520924085No Hit
CCCTTAACTTTATGGGATATGTAATTGGGAGTTGGGGTACTTTACCACAG7310.15405657733071162No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7250.1527920910598713No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7070.14899863224735038No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6790.14309769631676222No Hit
ATGTAATTGGGAGTTGGGGTACTTTACCACAGGAACACATTGTACAAAAA6730.14183321004592192No Hit
ACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACAT6410.1350892832681069No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6300.132771058438233No Hit
CCCTTAGACAAGGGCATTAAACCTTACTATCCGGAACATGCAGTTAATCA6110.12876685191390536No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5930.1249733931013844No Hit
ACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATGTTAGG5930.1249733931013844No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5880.12391965454235082No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5780.12181217742428363No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCC5680.11970470030621641No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5670.1194939525944097No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA5630.11865096174718283No Hit
CTCCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5630.11865096174718283No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5590.11780797089995597No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTTCGAAAAC5580.11759722318814922No Hit
CCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5490.11570049378188875No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5430.11443600751104846No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5430.11443600751104846No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA5390.11359301666382157No Hit
GTACAACACCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5390.11359301666382157No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAGTAAGGTTTAATGCC5370.11317152124020813No Hit
GGATTAAAGACAGGTATAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC5330.11232853039298127No Hit
GGTCTATTTACAGGAAGTTTTCGAAAACATTGTTTGAGTTTTTGTACAAT5280.11127479183394766No Hit
GTAATTGGGAGTTGGGGTACTTTACCACAGGAACACATTGTACAAAAACT5110.10769208073323343No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5080.10705983759781328No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG5060.10663834217419985No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATG4970.10474161276793938No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG4970.10474161276793938No Hit
GGGTAGAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4900.10326637878529234No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTATACCTGTC4850.10221264022625873No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4790.10094815395541844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)