FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452131
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT28960.6405223264938701No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA24040.5317043069375911No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC21840.48304584290836067No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20740.4587166108937454No Hit
ACTACATACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA19020.42067453901634705No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT18450.40806757333604643No Hit
CCCTTACAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTAGG17100.37820897040901863No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT15050.3328681289272357No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14300.316280016189998No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14180.31362591815204No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA13620.3012401273082359No Hit
ATGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTA12440.2751414966016486No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT12330.2727085734001871No Hit
GTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAA12250.2709391747082151No Hit
TTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT11570.255899285826453No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11550.25545693615345993No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11040.24417701949213835No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC10530.23289710283081674No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAATCCCCCATG10480.23179122864833424No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10120.22382893453446015No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGTATGTAGTTAATCATTAC9790.21653016493007557No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9770.21608781525708254No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9520.21055844434467003No Hit
GTTGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG9410.2081255211432085No Hit
TGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA9390.2076831714702155No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9310.20591377277824346No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9310.20591377277824346No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT9220.20392319924977498No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9060.20038440186583092No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8620.19065270905998483No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTGCGGGTCACCATATT8480.1875562613490338No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8340.18445981363808275No Hit
GTTTTATCCCAAGGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC8080.1787092678891737No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACGG7850.17362224664975417No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7710.17052579893880312No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA7600.1680928757373416No Hit
ATATTAACGTTGACATAACTGACTACTGCTTCCCTGGATGCTGGATCTTC7560.1672081763913556No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG7540.1667658267183626No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7490.1656599525358801No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG7460.16499642802639058No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7430.16433290351690108No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7420.16411172868040458No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7390.16344820417091507No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA7370.16300585449792207No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7320.16189998031543956No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCACCTCTATG7280.16101528096945356No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT7020.1552647352205445No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG7010.15504356038404798No Hit
ATCCCAAGGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT6980.15438003587455848No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6930.15327416169207597No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6880.15216828750959346No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACGGCACTCAGGCA6860.15172593783660046No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6830.15106241332711096No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6790.15017771398112492No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6790.15017771398112492No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6780.14995653914462845No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6760.14951418947163544No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACATAGCGCC6750.14929301463513894No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6220.1375707483008243No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6120.1353589999358593No Hit
CTACATACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT6110.13513782509936278No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6090.1346954754263698No Hit
ACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6070.13425312575337678No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6060.13403195091688028No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6020.13314725157089427No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC6010.13292607673439777No Hit
TGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA5990.13248372706140477No Hit
GTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5910.13071432836943275No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5880.13005080385994325No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5860.12960845418695024No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5750.12717553098548873No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5730.12673318131249572No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCCAAGGTTACTAAATATTTGC5700.12606965680300622No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5590.1236367336015447No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC5530.12230968458256568No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5470.12098263556358667No Hit
CTTTATCTAAGGGCAAATATTTAGTAACCTTGGGATAAAACCTAGCAGGC5410.11965558654460766No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5410.11965558654460766No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5380.11899206203511814No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT5370.11877088719862162No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTA5340.11810736268913213No Hit
GCCCCTTACAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTA5310.11744383817964262No Hit
CCTTACAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTAGGT5270.11655913883365661No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC5250.11611678916066362No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5210.1152320898146776No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTG5210.1152320898146776No Hit
CAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTAGGTTTTAT5200.1150109149781811No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5200.1150109149781811No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5200.1150109149781811No Hit
ACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5150.11390504079569859No Hit
CTGTAGATCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTAT5140.11368386595920207No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5130.1134626911227056No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5110.11302034144971258No Hit
GTAGTCAGTTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATT5050.11169329243073357No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5000.11058741824825107No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC4980.11014506857525805No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4980.11014506857525805No Hit
ATCTAAGGGCAAATATTTAGTAACCTTGGGATAAAACCTAGCAGGCATAA4970.10992389373876155No Hit
AAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAATA4940.10926036922927204No Hit
TCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT4920.10881801955627904No Hit
AGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4890.10815449504678953No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGTATGTAG4850.10726979570080354No Hit
AGATAAAGGGATCAAACCTTATTATCCAGAGTATGTAGTTAATCATTACT4840.10704862086430703No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGCAATTTGG4840.10704862086430703No Hit
CCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGTATGTAGTTAATCA4830.10682744602781052No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4830.10682744602781052No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4790.10594274668182452No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT4780.10572157184532802No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCTG4710.1041733479898525No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA4700.10395217315335599No Hit
GAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCT4700.10395217315335599No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4700.10395217315335599No Hit
AATTATGCCTGCTAGGTTTTATCCCAAGGTTACTAAATATTTGCCCTTAG4690.10373099831685949No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT4650.10284629897087348No Hit
GTTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT4630.10240394929788048No Hit
GGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA4610.10196159962488749No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4580.10129807511539797No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAA4560.10085572544240498No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4560.10085572544240498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)