FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452131
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT29190.6456093477332897No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA21980.4861422906193117No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20660.4569472122017734No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC19870.4394744001185497No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT17640.39015241157982977No Hit
CCCTTACAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTAGG17600.3892677122338437No Hit
ACTACATACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA16650.36825610276667603No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14940.3304352057257742No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT14620.32335761095788607No Hit
GTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAA13960.30876007174911696No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12970.2868637629359632No Hit
ATGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTA12330.2727085734001871No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11610.25678398517243894No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA11280.24948521556805442No Hit
TTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT11220.24815816654907538No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10950.2421864459636698No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC10870.24041704727169783No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10400.2300218299563622No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAATCCCCCATG10380.22957948028336922No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10260.2269253822454112No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG10130.22405010937095662No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9890.21874191329504059No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9840.21763603911255808No Hit
GTTGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG9690.21431841656511055No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG9570.2116643185271525No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9400.20790434630671198No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT9230.20414437408627145No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9040.1999420521928379No Hit
TGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA8850.1957397302994044No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8390.18556568782056526No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACGG8330.18423863880158625No Hit
GTTTTATCCCAAGGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC8200.18136336592713176No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG7880.17428577115924368No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7770.17185284795778213No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGTATGTAGTTAATCATTAC7770.17185284795778213No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7610.1683140505738381No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTGCGGGTCACCATATT7590.1678717009008451No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7490.1656599525358801No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7490.1656599525358801No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7450.16477525318989408No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7440.16455407835339758No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7410.16389055384390805No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7240.16013058162346752No Hit
ATATTAACGTTGACATAACTGACTACTGCTTCCCTGGATGCTGGATCTTC7170.15858235776799204No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7150.158140008094999No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7110.157255308749013No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT7100.1570341339125165No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACGGCACTCAGGCA7000.15482238554755148No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCACCTCTATG6940.15349533652857247No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6850.15150476300010396No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6840.15128358816360743No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6800.15039888881762142No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCTG6720.14862949012564944No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA6660.14730244110667043No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACATAGCGCC6590.1457542172511949No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6520.14420599339571938No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6490.14354246888622987No Hit
CTACATACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT6370.14088837084827185No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6260.13845544764681034No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT6040.1335896012438873No Hit
ACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5980.13226255222490826No Hit
ATCCCAAGGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT5980.13226255222490826No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5970.13204137738841176No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5960.13182020255191526No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5840.12916610451395724No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5810.12850258000446774No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTA5780.12783905549497823No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCCAAGGTTACTAAATATTTGC5550.12275203425555867No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC5530.12230968458256568No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTG5500.12164616007307616No Hit
GCCCCTTACAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTA5490.12142498523657966No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5470.12098263556358667No Hit
CTTTATCTAAGGGCAAATATTTAGTAACCTTGGGATAAAACCTAGCAGGC5460.12076146072709017No Hit
CCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGTATGTAGTTAATCA5440.12031911105409715No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5410.11965558654460766No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC5390.11921323687161464No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC5370.11877088719862162No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5360.11854971236212515No Hit
CCTTACAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTAGGT5350.11832853752562864No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5350.11832853752562864No Hit
GTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5350.11832853752562864No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGCAATTTGG5330.11788618785263563No Hit
CTGTAGATCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTAT5320.11766501301613912No Hit
GTAGTCAGTTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATT5250.11611678916066362No Hit
AATTATGCCTGCTAGGTTTTATCCCAAGGTTACTAAATATTTGCCCTTAG5220.1154532646511741No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5210.1152320898146776No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5190.11478974014168461No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAA5180.11456856530518811No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5150.11390504079569859No Hit
TGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA5140.11368386595920207No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5130.1134626911227056No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5100.11279916661321608No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5090.11257799177671957No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5040.11147211759423706No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4950.10948154406576856No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG4790.10594274668182452No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4790.10594274668182452No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA4750.1050580473358385No Hit
CAGTAAATGAAAACAGGAGACTGAAATTAATTATGCCTGCTAGGTTTTAT4740.104836872499342No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4730.10461569766284551No Hit
ACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4720.104394522826349No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGTATGTAG4710.1041733479898525No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4680.103509823480363No Hit
AAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAATA4680.103509823480363No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG4600.10174042478839097No Hit
ATCTAAGGGCAAATATTTAGTAACCTTGGGATAAAACCTAGCAGGCATAA4560.10085572544240498No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4530.10019220093291546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)