FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435817
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT16640.38181163194643625No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCCGG16390.3760752793030102No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14870.34119825523098No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12500.28681763217130124No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10800.2478104341960043No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10770.24712207187879315No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10520.24138571923536714No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9560.21935812508461122No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9500.21798140045018896No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9380.21522795118134444No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA9260.21247450191249997No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9000.20650869516333692No Hit
CATTTAAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8960.20559087874038873No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8770.20123125073138498No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8740.2005428884141738No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8370.1920530865019033No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7950.1824160140609476No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7690.17645020731178454No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC7540.1730083957257289No Hit
ATTGACAACAGTGCCAGCAGCACCGCCTCCTGCCTCCACCAATCGGCAGT7460.17117276287983257No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7120.1633713232847732No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7000.16061787401592872No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCCGGATAATACGGTTTAATGCC6950.1594706034872435No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT6850.1571760624298731No Hit
CCTACTCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA6700.15373425084381748No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6670.15304588852660636No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6600.15143970978644705No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6260.14363827019138767No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6260.14363827019138767No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6200.1422615455569654No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6180.14180263734549134No Hit
CCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA5810.1333128354332208No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5700.1307888402701134No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA5610.12872375331848No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5590.12826484510700592No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCGGAACATGCAGTTAATCA5560.1275764827897948No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5560.1275764827897948No Hit
TTCTTCTACTGTCCCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTC5470.12551139583816143No Hit
CCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA5460.12528194173242438No Hit
CCGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5440.12482303352095031No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5440.12482303352095031No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5440.12482303352095031No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCC5380.12344630888652806No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5340.1225284924635799No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5330.12229903835784285No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5260.12069285961768357No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5180.11885722677178723No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT5170.11862777266605021No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5170.11862777266605021No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5170.11862777266605021No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTTAAATGTATA5150.11816886445457611No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5140.11793941034883909No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4980.11426814465704642No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4920.11289142002262417No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4890.11220305770541306No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4880.11197360359967601No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4810.11036742485951673No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT4780.10967906254230561No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4770.10944960843656856No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4670.10715506737919815No Hit
TCACCATACAGCTCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGA4670.10715506737919815No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4520.10371325579314253No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA4520.10371325579314253No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4470.10256598526445734No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAACCTAGCAGGCATAA4420.10141871473577213No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT4410.10118926063003508No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4360.10004199010134988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)