FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435817
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCCGG17190.3944316077619735No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT13540.3106808591679535No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11560.2652489462320194No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11040.2533173327336933No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9980.22899519752556693No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9870.2264712023624595No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8630.19801889325106642No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8320.19090581597321812No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8290.190217453656007No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8270.1897585454445329No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8150.18700509617568842No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCCGGATAATACGGTTTAATGCC7820.17943311068636605No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC7720.17713856962899566No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT7710.1769091155232586No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7670.17599129910031044No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7620.17484402857162526No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7330.16818985950525106No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7070.162224052756088No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA7050.1617651445446139No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6430.14753898998891737No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6300.14455608661433583No Hit
CATTTAAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6280.14409717840286176No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6080.13950809628812094No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6000.1376724634422246No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5960.13675464701927645No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5870.13468956006764307No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5720.13124774848158746No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5580.12803539100126887No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5400.12390521709800215No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5350.12275794656931695No Hit
TTCTTCTACTGTCCCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTC5270.12092231372342062No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5120.117480502137365No Hit
ATTGACAACAGTGCCAGCAGCACCGCCTCCTGCCTCCACCAATCGGCAGT4990.11449759876278347No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT4980.11426814465704642No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCC4910.11266196591688714No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA4850.11128524128246489No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4780.10967906254230561No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAACCTAGCAGGCATAA4720.10830233790788335No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4700.10784342969640927No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4610.1057783427447759No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4550.10440161811035364No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT4550.10440161811035364No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCTCTCAGGCA4540.10417216400461662No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4520.10371325579314253No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4510.10348380168740548No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4380.10050089831282395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)