FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355133
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12240.3446596064009822No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10740.3024219095381167No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAACCGTAT8540.2404732874725807No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7200.2027409449417542No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6710.18894329729988482No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6680.18809854336262752No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5580.15712423232985953No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5360.1509293701233059No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5360.1509293701233059No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5210.14670560043701936No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5060.1424818307507328No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4950.13938439964745603No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4870.13713172248143654No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4860.1368501378356841No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG4660.13121844492063536No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4280.12051822838204278No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAAC4020.11319702759247945No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT3970.11178910436371726No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCTTTGACATACT3890.10953642719769775No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3860.10869167326044045No Hit
CCTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3740.10531265751141121No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3670.10334156499114416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)