FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341410
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13330.39043964734483466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9680.2835300664889722No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9540.27942942503148704No Hit
CTTTAGGACATGGACATTGATCCATATAAAGAATTTGGAGCTTCTGTGGA8610.2521894496353358No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT6600.19331595442429922No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5620.1646114642219033No Hit
CTATACAATTCACCCAGTTAAACTATGCAAGTCATTTCTTAAAATATATT5540.16226824053191177No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAATACCCC5200.1523095398494479No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4840.1417650332444861No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCATACGG4750.13912890659324564No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4470.13092762367827537No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4340.12711988518203918No Hit
GCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCATACGGCAATCAGGCA4160.1218476318795583No Hit
GTGCTGGAGACTTTGGGGAGAAATGGAGAGGAAATGCTATCATGTACGGT4130.1209689229958115No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT3890.11393925192583697No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG3840.11247473711959227No Hit
GCTTTAGGACATGGACATTGATCCATATAAAGAATTTGGAGCTTCTGTGG3680.10778828973960926No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT3640.10661667789461352No Hit
CATTTAAACCCTCACAAAACGAAAAGATGGGGATATTCCCTTAACTTCAT3620.10603087197211564No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3570.10456635716587094No Hit
GTGCTGGAATCAGAGGAGAGGGAAAGAGGAATGGAGGTTTTCGCCCCAGG3550.10398055124337306No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3530.10339474532087518No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3440.10075861866963476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)