FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402391
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC19620.4875854579252518No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19050.47342013116595555No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14290.3551272270006039No Hit
ATCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC11200.278336245094945No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT10220.25398182364913724No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9910.2462778740081165No Hit
AAGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC8530.2119828723803465No Hit
CCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA8490.21098881436215025No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7840.19483537156646147No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7780.19334428453916713No Hit
CATTTAAACCCGCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7690.1911076539982256No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7340.18240964633900858No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7080.17594826922073306No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7000.1739601531843406No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6770.16824431957971228No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6140.15258790579312162No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6110.15184236227947445No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6070.1508483042612782No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT5850.14538098516119893No Hit
GTTATACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT5810.14438692714300272No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT5780.14364138362935552No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5750.14289584011570836No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5720.1421502966020612No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5680.14115623858386495No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC5650.14041069507021778No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5640.14016218056566873No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5480.1361859484928838No Hit
ACCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5460.13568891948378567No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5430.1349433759701385No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5390.1339493179519423No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5380.1337008034473932No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5320.13220971642009888No Hit
ATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTCCATGC5300.13171268741100076No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5280.13121565840190264No Hit
GGGAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGG5210.12947605687005923No Hit
ATGTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5190.1289790278609611No Hit
AAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5060.12574833930182333No Hit
CTTTATGCCCATGTTAACGTTGACATAGCTGACTACTAATTCCCTGGATG5000.124257252274529No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCAATGAGTTT4980.12376022326543087No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4950.1230146797517837No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT4950.1230146797517837No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4930.12251765074268559No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCAATG4920.12226913623813652No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4920.12226913623813652No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4900.12177210722903842No Hit
GTCTAACTTTTGGGAGAGAAACTGTTCTTGAATATTTGGTGTCTTTTGGA4850.12052953470629313No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACGGGAACCTGCCC4850.12052953470629313No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4840.12028102020174407No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4820.11978399119264595No Hit
GCGTTCTATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGT4780.11878993317444972No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4780.11878993317444972No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGCGGGTTTAAATGTATAC4770.11854141866990066No Hit
GTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT4760.1182929041653516No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG4750.11804438966080255No Hit
CATTAACATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4690.1165533026335082No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC4660.11580775911986102No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4650.11555924461531196No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4590.11406815758801761No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4570.1135711285789195No Hit
GCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCTTGAGG4560.11332261407437044No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4550.11307409956982138No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4510.11208004155162515No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA4480.11133449803797799No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4460.11083746902887986No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTTTA4450.1105889545243308No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCCGC4410.10959489650613458No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4400.10934638200158552No Hit
CCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCAA4400.10934638200158552No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4390.10909786749703645No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4370.10860083848793835No Hit
CATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4290.10661272245154588No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAACCTCCTCC4260.10586717893789871No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4220.10487312091970247No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4190.1041275774060553No Hit
CTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4170.10363054839695718No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGGTTT4150.10313351938785907No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4080.10139391785601566No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4060.10089688884691754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)