FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402391
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18280.454284514315678No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18150.4510538257565403No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14170.3521450529460152No Hit
ATCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9680.24056204040348814No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9650.23981649688984097No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT9340.23211254724882016No Hit
CCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA8650.2149650464349352No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7860.1953324005755596No Hit
AAGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC7580.18837399444818598No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6920.17197203714794815No Hit
CATTTAAACCCGCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6910.17172352264339907No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6890.17122649363430095No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6850.17023243561610474No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6730.16725026156151604No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT6670.1657591745342217No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6340.15755819588410278No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6220.15457602182951408No Hit
GTTATACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT6070.1508483042612782No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5910.1468720721884933No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5810.14438692714300272No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5790.1438898981339046No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTTTA5780.14364138362935552No Hit
GTCTAACTTTTGGGAGAGAAACTGTTCTTGAATATTTGGTGTCTTTTGGA5700.14165326759296307No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5350.13295525993374605No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5280.13121565840190264No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5190.1289790278609611No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT5160.12823348434731394No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4990.12400873776997993No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4980.12376022326543087No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT4940.12276616524723465No Hit
ATGTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4890.12152359272448936No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4860.12077804921084218No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4850.12052953470629313No Hit
GTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT4820.11978399119264595No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4800.11928696218354784No Hit
AAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4790.11903844767899878No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4720.11729884614715537No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4690.1165533026335082No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4680.11630478812895914No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT4680.11630478812895914No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4620.11481370110166479No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG4600.11431667209256667No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4560.11332261407437044No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4550.11307409956982138No Hit
ACCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4540.11282558506527232No Hit
CTTTATGCCCATGTTAACGTTGACATAGCTGACTACTAATTCCCTGGATG4520.11232855605617421No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4500.11183152704707609No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCCGC4470.11108598353342893No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4450.1105889545243308No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4440.11034044001978174No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4440.11034044001978174No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4430.1100919255152327No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4410.10959489650613458No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACGGGAACCTGCCC4410.10959489650613458No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC4400.10934638200158552No Hit
GGGAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGG4210.10462460641515342No Hit
CATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4200.10437609191060436No Hit
CCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCAA4180.10387906290150624No Hit
GCGTTCTATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGT4160.10338203389240813No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4150.10313351938785907No Hit
CATTAACATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4150.10313351938785907No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4090.10164243236056472No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4070.1011454033514666No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4060.10089688884691754No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAACCTCCTCC4030.10015134533327037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)