FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445368
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37980.8527779274667241No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA28360.636776777855616No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT22900.5141815307790412No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC20290.4555783082754037No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAGTTTGTAGGCCCACT16920.37991054588564965No Hit
TTCTAATACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14750.33118679384239547No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14440.32422625783621634No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT10970.2463131612509206No Hit
GGATAAGGGAATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10750.24137342602072892No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC9750.21892008406531227No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAATCTAGCAGG9510.21353128199601226No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT9120.20477447863339981No Hit
GGATAATAAGGTTTAATTCCCTTATCCAATGGCAAATATTTGGTAACCTT9100.20432541179429145No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTAT8610.19332327423613732No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA8240.18501553771263315No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGAATTAAACCTTAT8110.182096603258429No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTATCC7790.17491153383269567No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA7390.165930197050529No Hit
GATTATGCCTGCTAGATTTTATCCAAAGGTTACCAAATATTTGCCATTGG7350.16503206337231233No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACTACCGTG7300.16390939627454149No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7250.16278672917677067No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7150.160541394981229No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7120.15986779472256651No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT7110.15964326130301235No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATGCGTTGACACACT7110.15964326130301235No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTATCCAAAGGTTA6680.14998832426218317No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6640.14909019058396653No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAGAGCAGGATACCCACAT6480.14549765587109983No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT6250.14033338722135402No Hit
GTTCTGGATAATAAGGTTTAATTCCCTTATCCAATGGCAAATATTTGGTA6140.1378635196062582No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6080.1365163190889332No Hit
CACCAAGACATTATTAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT6040.13561818541071655No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5940.13337285121517486No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5750.1291067162436457No Hit
GTATTAGAATATTTGGTGTCGTTCGGAGTGTGGATTCGCACTCCTCCAGC5720.1284331159849832No Hit
GTTTAATTCCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAT5620.12618778178944154No Hit
GTATTATGTGTTCTTGTGGCAATGACCCATGACATCCAATGACATAGCCC5570.1250651146916707No Hit
GGATACCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5530.12416698101345404No Hit
TTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG5480.1230443139156832No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG5480.1230443139156832No Hit
GTATATGGTGACCCACAAAATGAGGCACTATGTGTTGTTTCCCTCTTATA5380.12079897972014153No Hit
GCTTCCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGT5370.12057444630058738No Hit
AGTATATGGTGACCCACAAAATGAGGCACTATGTGTTGTTTCCCTCTTAT5330.1196763126223707No Hit
CTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATT5320.11945177920281655No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT5320.11945177920281655No Hit
ATATTTGCCATTGGATAAGGGAATTAAACCTTATTATCCAGAACATCTAG5320.11945177920281655No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTG5280.11855364552459988No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT5280.11855364552459988No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5130.11518564423128738No Hit
AGTCTGTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5070.11383844371396239No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4990.11204217635752906No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4960.11136857609886656No Hit
GAATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4900.11002137558154156No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4880.10957230874243322No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4880.10957230874243322No Hit
CCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4870.10934777532287905No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGT4790.10755150796644573No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTCATCTCCTCC4780.10732697454689155No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT4760.10687790770778323No Hit
GGTTACCAAATATTTGCCATTGGATAAGGGAATTAAACCTTATTATCCAG4600.10328537299491655No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGTATTATGTGTT4600.10328537299491655No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGGACAACAGAGT4590.1030608395753624No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATTTACAC4580.10283630615580822No Hit
ACTAAGTCCCTAGACGCTGGATCTTCCAAATTAGCACCCACCCAGGTAGC4560.10238723931669991No Hit
CCATATACTTGGGAACAAGATCTACAGCATGGGGCAGAATCTTTCCACCA4560.10238723931669991No Hit
ATGTCATTGGATGTCATGGGTCATTGCCACAAGAACACATAATACAAAAA4550.10216270589714573No Hit
GCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAA4540.10193817247759157No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4500.1010400387993749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)