FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445368
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37460.8411021896499074No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA28020.6291426415907744No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT23180.5204684665265578No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC17980.40371108835839126No Hit
TTCTAATACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA15720.35296653553914964No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAGTTTGTAGGCCCACT15630.35094573476316215No Hit
GGATAAGGGAATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10780.24204702627939143No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10280.2308203553016831No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAATCTAGCAGG9930.2229616856172873No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC9570.2148784825133373No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA9470.21263314831779564No Hit
GGATAATAAGGTTTAATTCCCTTATCCAATGGCAAATATTTGGTAACCTT9230.20724434624849564No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT9230.20724434624849564No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT9170.20589714573117066No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8670.1946704747534623No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA8550.1919760737188123No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTATCC8470.19017980636237897No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATGCGTTGACACACT8110.182096603258429No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACTACCGTG8020.18007580248244148No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7910.17760593486734566No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTAT7640.17154353253938318No Hit
GATTATGCCTGCTAGATTTTATCCAAAGGTTACCAAATATTTGCCATTGG7390.165930197050529No Hit
GCTTCCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGT7070.15874512762479567No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGAATTAAACCTTAT7060.1585205942052415No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6650.14931472400352067No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT6630.14886565716441233No Hit
GTTCTGGATAATAAGGTTTAATTCCCTTATCCAATGGCAAATATTTGGTA6510.14617125612976237No Hit
GTATTAGAATATTTGGTGTCGTTCGGAGTGTGGATTCGCACTCCTCCAGC6500.14594672271020817No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTATCCAAAGGTTA6430.14437498877332902No Hit
GTATATGGTGACCCACAAAATGAGGCACTATGTGTTGTTTCCCTCTTATA6330.14212965457778734No Hit
GGATACCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6280.14100698748001653No Hit
GTATTATGTGTTCTTGTGGCAATGACCCATGACATCCAATGACATAGCCC6180.13876165328447485No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6160.13831258644536654No Hit
GTTTAATTCCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAT6030.13539365199116235No Hit
CACCAAGACATTATTAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT5990.1344955183129457No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT5910.1326992509565124No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG5900.1324747175369582No Hit
AGTCTGTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5820.13067845018052487No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5800.13022938334141654No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT5750.1291067162436457No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAGAGCAGGATACCCACAT5740.12888218282409153No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5630.1264123152089957No Hit
TTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG5510.12371791417434572No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5490.12326884733523738No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTG5460.12259524707657489No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5330.1196763126223707No Hit
CTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATT5160.11585924448994989No Hit
ATATTTGCCATTGGATAAGGGAATTAAACCTTATTATCCAGAACATCTAG5100.11451204397262488No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5050.11338937687485405No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT5040.1131648434552999No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4990.11204217635752906No Hit
GCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAA4980.11181764293797487No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4960.11136857609886656No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT4900.11002137558154156No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4880.10957230874243322No Hit
AGTATATGGTGACCCACAAAATGAGGCACTATGTGTTGTTTCCCTCTTAT4870.10934777532287905No Hit
GGTTACCAAATATTTGCCATTGGATAAGGGAATTAAACCTTATTATCCAG4860.1091232419033249No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4740.10642884086867489No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGGACAACAGAGT4710.1057552406100124No Hit
ATGTGGTATTGGGGGCCAAGTCTGTATCGCATCTTGAGTCCCTTTTTACC4620.1037344398340249No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATTTACAC4610.10350990641447073No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT4580.10283630615580822No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT4530.10171363905803739No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTATCGCATCTTG4460.10014190512115824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)