FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384951
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA35830.9307678120072425No Hit
CTCCTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT16880.4384973671973835No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT16200.42083278131502455No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA15260.3964140890658811No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12760.33147075861603165No Hit
CCTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11970.31094866619387923No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA11130.2891277071627298No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10700.27795745432535574No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC9760.25353876207621234No Hit
GTGTAGTTTCTCTCCTATATAATATACCCGCCTTCCATAGAGTGTGTAAA9620.24990193557102072No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9470.24600533574402977No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9130.23717304280285023No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG8790.22834074986167072No Hit
GTTTTATCCAAAGGTAACCAAATATTTGCCATTAGATAAGGGCATTAAAC8450.21950845692049117No Hit
CTCTGATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8340.2166509503806978No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8270.21483253712810202No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTACTCTTTACATTTCAT8250.21431299048450322No Hit
GGATAATAAGGTTTAATGCCCTTATCTAATGGCAAATATTTGGTTACCTT7900.20522092422152427No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7810.2028829643253297No Hit
AGATAAGGGCATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7780.20210364435993153No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7660.19898636449833876No Hit
CCTCCAGCTTATAGGCCAGTAAATGCCCCTATCTTATCGACACTTCCGGA7470.1940506713841502No Hit
ATCCAAAGGTAACCAAATATTTGCCATTAGATAAGGGCATTAAACCTTAT7430.1930115780969526No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACCACCGTG7370.19145293816615622No Hit
AAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTAA7270.18885520494816224No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7250.18833565830456345No Hit
AAAACGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTAA7240.18807588498276406No Hit
CTCTTCTACTGTGCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7120.18495860512117127No Hit
CTCTATGGAAGGCGGGTATATTATATAGGAGAGAAACTACACATAGCGCC6950.1805424586505815No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTAACCAAATATTTGCCATTAG6790.17638608550179113No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6560.17041129910040498No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGT6550.1701515257786056No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6390.1659951526298152No Hit
GCATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6200.16105945951562667No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5880.15274671321804592No Hit
GTTAGTATTCCGTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC5590.1452132868858634No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5490.14261555366786943No Hit
CTTCTACTGTGCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5190.13482235401388748No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5100.1324843941176929No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTAACCAAATATTTGC4970.1291073409343007No Hit
GTTCTGGATAATAAGGTTTAATGCCCTTATCTAATGGCAAATATTTGGTT4960.12884756761250132No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4910.12754870100350435No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCACGGAAT4880.12676938103810617No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4820.12521074110730976No Hit
GTTAGCATTCCGTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC4820.12521074110730976No Hit
ATGTGTAGTTTCTCTCCTATATAATATACCCGCCTTCCATAGAGTGTGTA4790.12443142114191158No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4780.12417164782011217No Hit
CTCTAAAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4770.12391187449831277No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4760.12365210117651337No Hit
CCCTTATCTAATGGCAAATATTTGGTTACCTTTGGATAAAACCTAGCAGG4750.12339232785471399No Hit
CTTTTACACAATGTGGTTATCCTGCTTTGATGCCTTTGTATGCATGCATT4680.1215739146021182No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4670.1213141412803188No Hit
ATATAGGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT4600.119495728027723No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA4590.1192359547059236No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAAA4380.11378071494813627No Hit
GTCTCTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4330.11248184833913928No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTGCCTGTC4220.10962434179934588No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4220.10962434179934588No Hit
ATATTAGTGTTAACATAAGTGACTACTAGGTCTCTGGATGTTGGATCTTG4200.1091047951557471No Hit
GGTTTAATGCCCTTATCTAATGGCAAATATTTGGTTACCTTTGGATAAAA4110.10676683525955252No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4080.10598751529415432No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4030.10468864868515733No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT4020.10442887536335793No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4010.10416910204155855No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCAGAGC4000.10390932871975912No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4000.10390932871975912No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTTTCATTGAC3980.10338978207616034No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3950.10261046211076214No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC3920.10183114214536396No Hit
AGTCTGTATCGCATCTTGAGTCCCTTCTTACCGCTGTTACCAATTTTCTT3920.10183114214536396No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA3890.10105182217996576No Hit
CTTCTTTTTTCATTGACTGTGAGTGGGCCTACAAATTGTTCACATTTTTT3870.10053227553636697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)