FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424490
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG23190.5463026219698933No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14450.3404084901882259No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12030.28339890221206626No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11820.2784517892058706No Hit
GTACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG11140.2624325661381893No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10680.25159603288652266No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAC10560.24876911116869654No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10440.24594218945087043No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9710.22874508233409505No Hit
TAGACAAAGGCATTAAACCTTACTATCCGGAACATGCAGTTAATCATTAC9090.21413932012532685No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG9010.21225470564677612No Hit
ATCTTAACCTCTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG8680.20448067092275435No Hit
CCCTTAACTTTATGGGATATGTGATTGGGAGTTGGGGTACTTTACCACAG8550.20141817239510942No Hit
ACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACAT8220.19364413767108765No Hit
CTACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC8030.18916817828452967No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7950.18728356380597894No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA7890.18587010294706588No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAACCACATTGTGTAAAA7730.1821008739899644No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7550.17786049141322527No Hit
CCTCTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7350.17314895521684845No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7180.16914414944992814No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7030.16561049730264552No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6940.16349030601427594No Hit
GGGTAGAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6890.16231242196518175No Hit
GGGTAAAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6620.15595184810007304No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6490.1528893495724281No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCC6330.14912012061532662No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT6310.14864896699568894No Hit
TACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATGTGAG6260.14747108294659472No Hit
TAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTA6050.14252396994039906No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG6000.14134608589130487No Hit
GGTTAGGATAGAACCTAGCAGGCATTATTAATTTTAATCTCCTTTTTTCA6000.14134608589130487No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5960.1404037786520295No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT5830.13734128012438457No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCTTTGTATGCATGTATA5770.1359278192654715No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5640.1328653207378266No Hit
CCCATAGGTATCTTGCGAAAGCCAAAGATGATGGGATGGGAATACAAGTG5560.13098070625927583No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5540.13050955263963815No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5520.13003839902000047No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA5520.13003839902000047No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5420.12768263092181206No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5340.12579801644326133No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5340.12579801644326133No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5260.1239134019647106No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG5150.12132205705670333No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACGTTCCACCAAGCTCTGCTAG5150.12132205705670333No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5140.1210864802468845No Hit
TACAAATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATGTGAG4910.11566821362105115No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4870.11472590638177578No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4860.11449032957195693No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG4820.11354802233268158No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4750.11189898466394967No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4750.11189898466394967No Hit
GGATTAAAGACAGGTATAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4730.111427831044312No Hit
GTACTAAAACTCAAACAATGTTTTCGAAAACTTCCTGTAAATAGACCTAT4730.111427831044312No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4720.11119225423449315No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA4690.11048552380503664No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTATACCTGTC4640.10930763975594243No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAGTAAGGTTTAATGCC4640.10930763975594243No Hit
CAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4640.10930763975594243No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4590.10812975570684823No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4570.10765860208721054No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4530.10671629484793517No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC4530.10671629484793517No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4530.10671629484793517No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4520.10648071803811632No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4450.10483168036938444No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4440.1045961035595656No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC4320.10176918184173951No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4320.10176918184173951No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCGTCTCG4310.10153360503192066No Hit
GTGGTTATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG4300.10129802822210182No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCGTCTCGCGGCCG4290.10106245141228297No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4290.10106245141228297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)