FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531845
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26720.5024020156248531No Hit
CTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT23460.44110596132331786No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20020.37642546230574697No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT17490.32885521157480097No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGATTAATGAAAAA15690.29501076441444407No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT12910.24273989602233734No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA12040.22638174656149818No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10780.20269063354924838No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10750.2021265594299091No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10600.1993061888332127No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10500.19742594176874845No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10350.19460557117205202No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10300.19366544763981988No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGGTAGGAAAGGAGGGAGTTTG9920.18652050879485566No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9680.1820079158401414No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9540.17937556994989143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9540.17937556994989143No Hit
CTCTTACGATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9520.17899952053699855No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9410.17693124876608785No Hit
ATATTATACAGTACGGTGGCCAGAGTTAATATAACTTGAAATTTGCGAAG8700.16358149460839155No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA8530.16038507459880227No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAT8260.15530840752474875No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8200.15418025928607018No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8080.15192396280871306No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8010.15060778986358808No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7820.14703532044110595No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7600.14289877689928457No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC7360.1383861839445703No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7060.1327454427511775No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7020.13199334392539178No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6940.13048914627382038No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTGAGTACAATA6940.13048914627382038No Hit
TATTTAAGGTGTACAACGTGATGATTTATTTTGTGAACTGATGACACAAT6920.1301130968609275No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6800.1278568003835704No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC6790.12766877567712398No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTACCATTCATTTACAGG6780.12748075097067754No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6760.1271047015577847No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6730.1265406274384454No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTGAGT6330.11901963918058832No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6320.1188316144741419No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTACC6220.11695136740967763No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC6200.11657531799678478No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTGAGTACAAT6190.11638729329033835No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA6100.11469507093232051No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6030.11337889798719553No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6020.11319087328074909No Hit
GTTAACATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5990.11262679916140982No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5920.11131062621628483No Hit
ACGTTAATATGGGCATAAAACTCAGACAACTATTGTGGTTTCACATTTCC5780.10867828032603483No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATGCG5780.10867828032603483No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC5770.1084902556195884No Hit
CTTACGATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5770.1084902556195884No Hit
ATATTGTACTCAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA5760.10830223091314198No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAA5650.10623395914223129No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATC5620.105669885022892No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5610.10548186031644557No Hit
CAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATA5580.1049177861971063No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5540.10416568737132059No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACGATTAATGAAAAAAG5420.10190939089396348No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT5380.10115729206817776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)