FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531845
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26540.4990175709088174No Hit
CTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT22640.42568793539471084No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21500.4042531188598182No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT15990.3006515056078369No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGATTAATGAAAAA15420.28993409734039055No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT13280.24969681016085515No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA12850.24161174778365876No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11960.22487754890992678No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10940.20569902885239122No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10760.20231458413635553No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGGTAGGAAAGGAGGGAGTTTG10190.19159717586890918No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10070.18934087939155203No Hit
CTCTTACGATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10010.18821273115287349No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9920.18652050879485566No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9870.18558038526262352No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9850.18520433584973064No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA9620.18087976760146285No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9550.17956359465633787No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9500.1786234711241057No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9380.1763671746467486No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8690.16339346990194512No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8500.15982100047946302No Hit
ATATTATACAGTACGGTGGCCAGAGTTAATATAACTTGAAATTTGCGAAG8140.1530521110473916No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTGAGTACAATA8010.15060778986358808No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7910.1487275427991238No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC7700.14477902396374884No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7580.1425227274863917No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAT7580.1425227274863917No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7410.13932630747680244No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC7340.13801013453167746No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7330.13782210982523105No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTACCATTCATTTACAGG7200.1353777886414275No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7070.1329334674576239No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6840.12860889920935611No Hit
TATTTAAGGTGTACAACGTGATGATTTATTTTGTGAACTGATGACACAAT6800.1278568003835704No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA6760.1271047015577847No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6560.12334420742885616No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6430.12089988624505259No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAA6410.12052383683215974No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6380.11995976271282045No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6260.11770346623546334No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6150.11563519446455264No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6130.1152591450516598No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6120.11507112034521336No Hit
ATATTGTACTCAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA5970.11225074974851697No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5950.1118747003356241No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTACC5900.11093457680339197No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTGAGT5820.10943037915182054No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTGAGTACAAT5810.10924235444537411No Hit
ACTTAATACACATCTCTCACCTCATACAATTATCCGTTTTTTTGGAGATA5660.10642198384867771No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5550.10435371207776702No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC5400.10153334148107061No Hit
GTTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5370.10096926736173134No Hit
GTTAACATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5330.10021716853594563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)