FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519851
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68351.3147998176400546No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17960.34548360972663317No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT16800.3231695235750244No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTTTGGAAATAATGATTAACTACA15540.29893180930689756No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14540.27969552814171755No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9690.18639956449059442No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9680.1862072016789426No Hit
TTATTATCCAGAGCATGTAGTTAATCATTATTTCCAAACGAGACATTATT9100.17505015860313822No Hit
GCACCGGACCATGCAAAACCTGCACGACACCTGCTCAAGGAAACTCTATG8160.15696805430786898No Hit
ATGTATATTTGCGCGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTC7810.15023535590005596No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7760.14927354184179698No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7760.14927354184179698No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA7700.14811936497188619No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7490.14407974592719835No Hit
GTGTAAATAATGTCTCGTTTGGAAATAATGATTAACTACATGCTCTGGAT7010.13484633096791196No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6890.13253797722809035No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA6670.12830599537175075No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCCACACTTCCGGA6650.12792126974844714No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT6620.12734418131349173No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAA6080.11695658948429455No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5990.11522532417942834No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTTTGGA5960.11464823574447293No Hit
CCCTTACAGTAAATGAAACCCGGAGACTAAAATTGATTATGCCTGCTAGG5850.11253224481630314No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5840.11233988200465134No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5710.10983916545317793No Hit
TCACAGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5680.10926207701822253No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5650.10868498858326713No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTCAATATGGGCCTAAAAATCAGA5620.10810790014831172No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG5600.10772317452500814No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAT5520.10618427203179372No Hit
ATATAAAAGAGAGTCCACACGCAGCGCCTCATTTTGCGGGTCACCATATT5430.10445300672692753No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5400.10387591829197212No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5340.10272174142206134No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5240.10079811330554332No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5200.10002866205893611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)