FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366229
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG12540.34240871148925944No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT9760.2664999221798383No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9420.2572161134153767No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9300.2539394750279197No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8750.2389215490854083No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8330.22745331472930871No Hit
CTCTAACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG7830.21380065478157106No Hit
GTGCTGGGGCAGGCCTGAGCCCAGGGTTGCTGGGGCCAGCCCAGAGACCG7570.20670127160874754No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7520.20533600561397375No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7450.2034246332212905No Hit
GTGCTGGCATCTGCTCAGTTTCTAGGGAGGCCGCAGGCAATTAACAATCA7220.1971444096453312No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTAACAGTTAACG6770.18485701569236734No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6430.17557320692790576No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6080.16601634496448944No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC5890.16082833418434914No Hit
GTGCTGGGAGTGGCCCGGGGTGTTTTTCCATATTTGCTTTAGTGGAGTTC5800.15837085539375637No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT5770.1575516957968921No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCACG5520.1507253658230233No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5140.1403493442627427No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC4960.13543438668155716No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4830.1318846950951454No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA4720.1288811099066431No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4590.12533141832023134No Hit
ATATAAAACATACTGATGAAGAGCAATATGATGGAATAGGACTTTCCACT4510.12314699272859332No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4490.1226008863306838No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC4350.11877814154531727No Hit
CTGTTAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4340.11850508834636252No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT4200.11468234356099599No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4070.1111326519745842No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC4000.10922127958190093No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3990.10894822638294618No Hit
GTTTTATATTTATGACCAATGGTTTTAAAATATTGCAGGCATTTTTTTCA3970.10840211998503668No Hit
GTCCATAGGTACTACCTTGAATTTTGTGGTTGTGGTAGCCTGCTCAGTGT3940.10758296038817243No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCGATAA3930.10730990718921768No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3880.10594464119444393No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT3870.10567158799548917No Hit
ATATAAAACTGCCCGTCCTACTCTCTTTAATGCACCTTTTCTGATTTCTG3810.10403326880176066No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT3780.10321410920489638No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3760.10266800280698687No Hit
GAATAGGACTTTCCACTGTTCCCTCTTCAGAAACATCAATTTGAACAACA3740.10212189640907737No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3740.10212189640907737No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA3720.10157579001116787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)