FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366229
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG13850.378178680552332No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9200.25120894303837216No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8950.24438261306450335No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT8330.22745331472930871No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7940.21680423997007336No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7810.21325454838366156No Hit
CTCTAACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG7410.2023324204254715No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7030.1919563988651909No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6710.18321869649863884No Hit
GTGCTGGGGCAGGCCTGAGCCCAGGGTTGCTGGGGCCAGCCCAGAGACCG6300.172023515341494No Hit
GTGCTGGCATCTGCTCAGTTTCTAGGGAGGCCGCAGGCAATTAACAATCA6260.17093130254567498No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCACG6120.16710855776030845No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG5980.1632858129749419No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT5900.16110138738330387No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTAACAGTTAACG5800.15837085539375637No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5420.14799483383347578No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5210.14226071665542597No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT5180.1414415570585617No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC5170.14116850385960697No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4910.1340691206867834No Hit
ATATAAAACATACTGATGAAGAGCAATATGATGGAATAGGACTTTCCACT4770.13024637590141688No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4740.12942721630455262No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC4700.12833500350873359No Hit
CTGTTAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4570.12478531192232183No Hit
GTTTTATATTTATGACCAATGGTTTTAAAATATTGCAGGCATTTTTTTCA4560.12451225872336708No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4490.1226008863306838No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC4480.12232783313172906No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4480.12232783313172906No Hit
GTCCATAGGTACTACCTTGAATTTTGTGGTTGTGGTAGCCTGCTCAGTGT4380.11959730114218152No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC4230.11550150315786024No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT4150.11331707756622224No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCGATAA4080.11140570517353895No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4050.1105865455766747No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4000.10922127958190093No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA3970.10840211998503668No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT3850.10512548159757966No Hit
GTGCTGGGAGTGGCCCGGGGTGTTTTTCCATATTTGCTTTAGTGGAGTTC3850.10512548159757966No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT3850.10512548159757966No Hit
GAATAGGACTTTCCACTGTTCCCTCTTCAGAAACATCAATTTGAACAACA3840.10485242839862491No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3810.10403326880176066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)