FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400035
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT17670.44171135025685254No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15770.39421550614321244No Hit
GACCCATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAACCTAGTCT13470.3367205369530166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13400.3349706900646193No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT11580.2894746709662904No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11520.2879748022048071No Hit
CATTTAAACCCTACTAAAACTAAACGGTGGGGATACTCCCTTAACTTCAT10940.27347607084380116No Hit
ACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTG10930.27322609271688725No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT10620.26547677078255655No Hit
ATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT10510.2627270113865037No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAACCTAGTCT9240.230979789268439No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9040.2259802267301611No Hit
GTGCTGGTGTGTGCGGCGCCTGTTGGTGAACTATTGCAGAGCCTCCCAGC8930.22323046733410826No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8560.21398127663829417No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT7890.19723274213506317No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7840.1959828515004937No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7820.1954828952466659No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7680.19198320146987138No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7580.18948342020073242No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCG7420.1854837701701101No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7190.17973427325109054No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6870.1717349731898459No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC6800.16998512630144863No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6790.16973514817453472No Hit
GGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAACTTCCTATAA6780.16948517004762084No Hit
GCTTACTACTGCTTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC6770.16923519192070693No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6580.16448560750934293No Hit
ATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAAGT6360.15898608871723724No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6180.15448648243278712No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6100.15248665741747597No Hit
AGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGT6070.15173672303673427No Hit
AGTTAAGGGAGTATCCCCACCGTTTAGTTTTAGTAGGGTTTAAATGAATA6010.1502368542752509No Hit
GTGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA6000.14998687614833703No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5970.14923694176759533No Hit
CCTGCACCTTACAGACCACCAAATGCCCCTATCTTATCGACACTTCCGGA5780.14448735735623133No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5780.14448735735623133No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5710.14273751046783406No Hit
GGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAAT5660.14148761983326458No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG5530.13823790418338394No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5310.13273838539127827No Hit
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5290.13223842913745049No Hit
GTTAAGGGAGTATCCCCACCGTTTAGTTTTAGTAGGGTTTAAATGAATAC5250.13123851662979488No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5250.13123851662979488No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA5220.13048858224905321No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5100.12748884472608646No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5100.12748884472608646No Hit
GTTTAAATGAATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4800.11998950091866963No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG4800.11998950091866963No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4800.11998950091866963No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4790.11973952279175572No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4780.11948954466484182No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4780.11948954466484182No Hit
GTGTCGATAAGATAGGGGCATTTGGTGGTCTGTAAGGTGCAGGAGTGCGA4690.11723974152261676No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAGC4600.1149899383803917No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGT4560.11399002587273613No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG4420.11049033209594161No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGC4400.10999037584211381No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4340.10849050708063046No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4320.10799055082680266No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4320.10799055082680266No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA4310.10774057269988875No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGT4300.10749059457297486No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4290.10724061644606098No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4250.1062407039384054No Hit
CCTGCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4240.10599072581149149No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4220.1054907695576637No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAGGGAGC4210.10524079143074982No Hit
CAGAAACATATTGTACATAAAATCAAACAATGCTTTCGGAAACTTCCTAT4210.10524079143074982No Hit
CCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGT4200.10499081330383592No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCA4100.10249103203469696No Hit
ACATCGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACC4060.10149111952704139No Hit
CAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTATTT4030.1007411851462997No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4010.1002412288924719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)